biostudies-literatureBecker AMDEuropean Research CouncilDutch Research Council (NWO)101048https://www.ebi.ac.uk/biostudies/studies/S-EPMC12454896biostudies-literatureUnknown24(9)Dendritic cells (DCs) are professional antigen-presenting cells endowed with the capacity to initiate strong antitumor immune responses. This function is critical for effective DC-based immunotherapies but is often hampered by tumor-derived immunosuppressive factors, as is observed for CD14⁺CD163⁺ tumor-induced DC3s (ti-DC3s). ti-DC3s are increased in cancer patients where they display an immunosuppressive phenotype, accompanied by weak antigen-specific CD8 T cell-activating capacities. While tumor-derived interleukin-6, macrophage colony-stimulating factor, and prostaglandin E2 have been identified as factors inducing the transition from DC2s to ti-DC3s, a comprehensive unbiased profiling of the resulting changes in secretome and proteome has not been reported. Here,Molecular & cellular proteomics : MCPProteome and Secretome Profiling of the Melanoma-Induced Transition Toward Immune Incompetent Dendritic Cells Reveals Enhanced IDO1, Cathepsin, and Legumain Activity.PMC12454896024.002.009679921Ignacio BJZiegler ARVerdoes MRamos-Tomillero IBonger KMde Vries IJMvan Dalen FJEdgington-Mitchell LEBecker AMDVermeulen MDijkstra JJfalseProteome and Secretome Profiling of the Melanoma-Induced Transition Toward Immune Incompetent Dendritic Cells Reveals Enhanced IDO1, Cathepsin, and Legumain Activity.Dendritic cells (DCs) are professional antigen-presenting cells endowed with the capacity to initiate strong antitumor immune responses. This function is critical for effective DC-based immunotherapies but is often hampered by tumor-derived immunosuppressive factors, as is observed for CD14⁺CD163⁺ tumor-induced DC3s (ti-DC3s). ti-DC3s are increased in cancer patients where they display an immunosuppressive phenotype, accompanied by weak antigen-specific CD8 T cell-activating capacities. While tumor-derived interleukin-6, macrophage colony-stimulating factor, and prostaglandin E2 have been identified as factors inducing the transition from DC2s to ti-DC3s, a comprehensive unbiased profiling of the resulting changes in secretome and proteome has not been reported. Here,2025-01-01T00:00:00Z2025 Aug2026-06-03T19:54:13.909Z2026-05-01T03:10:47.16ZS-EPMC124548964078945210.1016/j.mcpro.2025.101048