{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Linden M"],"funding":["Vetenskapsrådet","•the Swedish state under the agreement between the Swedish government and the county councils, the ALF-agreement","Barncancerfonden","Cancerfonden"],"pagination":["107"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12455829"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30(1)"],"pubmed_abstract":["<h4>Background</h4>FET (FUS, EWSR1, and TAF15) fusion oncoproteins are characteristic for several sarcomas and leukemias, including myxoid liposarcoma and Ewing sarcoma. FET oncoproteins interact with the SWI/SNF chromatin remodeling complex subtypes cBAF, PBAF, and GBAF, but their impact on SWI/SNF compositions, interactions, and downstream epigenetic effects remains elusive.<h4>Methods</h4>We employ a comprehensive immunoprecipitation and quantitative mass spectrometry approach to determine the impact of FET oncoproteins on SWI/SNF composition and their interactomes. Validation of complex composition and interaction partners is performed by glycerol gradient sedimentation assays and co-immunofluorescence analysis. Furthermore, we determine the differential chromatin accessibility and gen"],"journal":["Cellular & molecular biology letters"],"pubmed_title":["FET fusion oncoproteins enrich SWI/SNF complex subtypes and interaction partners."],"pmcid":["PMC12455829"],"funding_grant_id":["965065","22-2470","1006009","2023-02239","966318","22-2080","2021-01008","D2025-0003","2022-0030"],"pubmed_authors":["Andersson L","Aman P","Sjovall D","Jaako P","Linden M","Jonasson E","Crescitelli R","Fagman H","Stahlberg A","Albatrok H","Canfjorden V","Gronqvist K"],"additional_accession":[]},"is_claimable":false,"name":"FET fusion oncoproteins enrich SWI/SNF complex subtypes and interaction partners.","description":"<h4>Background</h4>FET (FUS, EWSR1, and TAF15) fusion oncoproteins are characteristic for several sarcomas and leukemias, including myxoid liposarcoma and Ewing sarcoma. FET oncoproteins interact with the SWI/SNF chromatin remodeling complex subtypes cBAF, PBAF, and GBAF, but their impact on SWI/SNF compositions, interactions, and downstream epigenetic effects remains elusive.<h4>Methods</h4>We employ a comprehensive immunoprecipitation and quantitative mass spectrometry approach to determine the impact of FET oncoproteins on SWI/SNF composition and their interactomes. Validation of complex composition and interaction partners is performed by glycerol gradient sedimentation assays and co-immunofluorescence analysis. Furthermore, we determine the differential chromatin accessibility and gen","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T19:38:59.684Z","creation":"2026-04-30T03:12:31.648Z"},"accession":"S-EPMC12455829","cross_references":{"pubmed":["40988026"],"doi":["10.1186/s11658-025-00792-w"]}}