{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lee WH"],"funding":["A*STAR National Science Scholarship","NIEHS NIH HHS","National Institutes of Health","NIH HHS","NIGMS NIH HHS"],"pagination":["e0057625"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12455946"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(9)"],"pubmed_abstract":["<i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> cause debilitating polymicrobial infections in diverse patient populations. Studies of these bacterial pathogens in coculture have shown that environmental variables, including Fe availability and the host-defense protein calprotectin (CP), impact coculture dynamics. To decipher how CP modulates interactions between <i>P. aeruginosa</i> and <i>S. aureus</i>, we employed dual-species RNA-seq to examine the transcriptional responses of both pathogens in coculture to CP treatment and metal depletion. Analysis of these responses revealed that, for both <i>P. aeruginosa</i> and <i>S. aureus</i>, CP treatment not only induced gene expression changes consistent with single- and multi-metal starvation responses but also induced gene ex"],"journal":["mSystems"],"pubmed_title":["Calprotectin protects &lt;i&gt;Staphylococcus aureus&lt;/i&gt; in coculture with &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; by attenuating quorum sensing and decreasing the production of pseudomonal antimicrobials."],"pmcid":["PMC12455946"],"funding_grant_id":["P30 ES002109","R01 GM126376"],"pubmed_authors":["Nolan EM","Lee WH","Oglesby AG"],"additional_accession":[]},"is_claimable":false,"name":"Calprotectin protects &lt;i&gt;Staphylococcus aureus&lt;/i&gt; in coculture with &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; by attenuating quorum sensing and decreasing the production of pseudomonal antimicrobials.","description":"<i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> cause debilitating polymicrobial infections in diverse patient populations. Studies of these bacterial pathogens in coculture have shown that environmental variables, including Fe availability and the host-defense protein calprotectin (CP), impact coculture dynamics. To decipher how CP modulates interactions between <i>P. aeruginosa</i> and <i>S. aureus</i>, we employed dual-species RNA-seq to examine the transcriptional responses of both pathogens in coculture to CP treatment and metal depletion. Analysis of these responses revealed that, for both <i>P. aeruginosa</i> and <i>S. aureus</i>, CP treatment not only induced gene expression changes consistent with single- and multi-metal starvation responses but also induced gene ex","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-04T02:00:04.222Z","creation":"2026-05-31T03:06:37.513Z"},"accession":"S-EPMC12455946","cross_references":{"pubmed":["40905698"],"doi":["10.1128/msystems.00576-25"]}}