biostudies-literatureUnknown44(9)Sadek Mthe NARAUniversity of Fribourg, SwitzerlandUniversity of Fribourg<h4>Purpose</h4>A series of NDM-producing Escherichia coli ST167 clinical isolates exhibiting resistance to cefiderocol (FDC), with no previous exposure to this antibiotic, were analyzed in this study.<h4>Methods</h4>The antimicrobial susceptibility testing and phenotypic detection of resistance patterns (Rapid Cefiderocol NP test and MIC determination) were performed for all tested isolates. Their entire genomes were sequenced by using the Illumina MiSeq platform and high-quality reads were de-novo assembled using the CLC Genomic Workbench. Genome-sequence based characteristics were analyzed using bioinformatics tools.<h4>Results</h4>All NDM-producing E. coli ST167 isolates showed a high level of resistance to FDC (MICs being 64 or > 64 mg/L). The chromosomally located cirA gene, encodingEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2059-2066https://www.ebi.ac.uk/biostudies/studies/S-EPMC12457464biostudies-literatureCombined resistance mechanisms leading to high-level of cefiderocol resistance among NDM-like producing E. coli ST167 clinical isolates.PMC12457464Lemaire BMonnerat LBBoschung DFankhauser HGreub GDubois OMoraz MPranghofer SCasanova CElzi MGuler CTrachsel SMaffioli CGoldenberger DEmonet SHitz ENarr KPozzi LLiassine NDimitrijevic DSuter BMueller GLWampfler RMathis BBlanco MMBandeira DCirillo CStaehli PKeller PJayol ASchmid SSchultze DOberle MScherler AHerzog KMitrovic IVonallmen LSchmid TMartinotti LWohlwend NGraf SBurren KLienhard RAssman HPayen CNARA NetworkGraff KImhof AJutzi MFournier CPoirel LDubey NVitale AMaitrejean MFricker CVogel MBlanc DMaret RKuegler MMarti COSchoenenberger Mvon Arb BLucke KMaurer JRumebe LBasilico LSchoch SChakraborty TErgani ADroz SHyden DLang CSchnell BSchibli UAndreutti CKurmann CGras PATogni GImperiali MElmer BAerni MPiran FBruderer VWesters AZowa CBelo AGruner EWehrli MCapaul RFatoux MDescombes MCGuyon CRosselin MJost GVaninetti GSeiffert SBalzari DEgli ACastelberg CGisler VSteffen IHinrikson HPianezzi ESimonet TDeggi-Messmer VAndrey DTritten MDi Lorenzo VPovolo VEllenberger SRenzi GDuran JBNusbaumer CZehnder CBrandenberger MSadek MSlutter VImeri FCherkaoui ASchrenzel JNordmann PComte LPreiswerk BSchilt CIvan BGuillod YKarrer UDessauges MMancini SBlaich AReichmuth MGaia VMabillard DEyer MMinkova PNguyen ASchacher MfalseCombined resistance mechanisms leading to high-level of cefiderocol resistance among NDM-like producing E. coli ST167 clinical isolates.<h4>Purpose</h4>A series of NDM-producing Escherichia coli ST167 clinical isolates exhibiting resistance to cefiderocol (FDC), with no previous exposure to this antibiotic, were analyzed in this study.<h4>Methods</h4>The antimicrobial susceptibility testing and phenotypic detection of resistance patterns (Rapid Cefiderocol NP test and MIC determination) were performed for all tested isolates. Their entire genomes were sequenced by using the Illumina MiSeq platform and high-quality reads were de-novo assembled using the CLC Genomic Workbench. Genome-sequence based characteristics were analyzed using bioinformatics tools.<h4>Results</h4>All NDM-producing E. coli ST167 isolates showed a high level of resistance to FDC (MICs being 64 or > 64 mg/L). The chromosomally located cirA gene, encoding2025-01-01T00:00:00Z2025 Sep2026-07-15T06:45:34.545Z2026-06-30T03:22:26.747ZS-EPMC124574644042372310.1007/s10096-025-05166-w