biostudies-literatureUnknown15(1)Ahmed MMLegume Seeds of Vicia are cultivated and consumed worldwide for their nutritional value and bioactive compounds. Notably, Vicia faba (fava bean) seeds, with their many cultivars or varieties, are deeply rooted in cuisines of the Middle East and across the globe. In this work, simple and fast spectroscopic techniques, including UV and FT-IR spectroscopy, were used in combination with multivariate statistical techniques not only to discriminate among different varieties of fava beans but also to distinguish them from other Vicia legumes, such as Vicia sativa and Vicia monantha. In addition, the total phytochemical phenolics and flavonoids, and in vitro radical scavenging activity were assessed. Preliminary exploratory data analysis using PCA on both UV and FT-IR spectra was capable of distinScientific reports32652https://www.ebi.ac.uk/biostudies/studies/S-EPMC12457650biostudies-literatureUsing UV and FTIR spectroscopy for discrimination among vicia seeds with emphasis on UV based multivariate modelling.PMC12457650Ismail YDonia AERMEl-Ahmady SHAhmed MMYoussef FSfalseUsing UV and FTIR spectroscopy for discrimination among vicia seeds with emphasis on UV based multivariate modelling.Legume Seeds of Vicia are cultivated and consumed worldwide for their nutritional value and bioactive compounds. Notably, Vicia faba (fava bean) seeds, with their many cultivars or varieties, are deeply rooted in cuisines of the Middle East and across the globe. In this work, simple and fast spectroscopic techniques, including UV and FT-IR spectroscopy, were used in combination with multivariate statistical techniques not only to discriminate among different varieties of fava beans but also to distinguish them from other Vicia legumes, such as Vicia sativa and Vicia monantha. In addition, the total phytochemical phenolics and flavonoids, and in vitro radical scavenging activity were assessed. Preliminary exploratory data analysis using PCA on both UV and FT-IR spectra was capable of distin2025-01-01T00:00:00Z2025 Sep2026-06-03T18:51:44.909Z2026-04-30T03:11:32.01ZS-EPMC124576504098778010.1038/s41598-025-17113-y