biostudies-literatureUnknown32(5)Charoenthanakitkul DAberrant extracellular matrix (ECM) production by dermal fibroblasts drives fibrotic skin diseases, which has an adverse impact on the lives of patients. Current treatments are limited; therefore, the development of new antifibrotic strategies is necessary. The aim of the present study was to investigate zinc finger 469 (ZNF469) as a potential ECM regulator in skin fibrosis. ZNF469 was knocked down in dermal fibroblasts using doxycycline‑induced short hairpin RNA. ZNF469 knockdown was found to impair proliferation, migration, contraction and collagen production in dermal fibroblasts. In addition, RNA sequencing revealed that ZNF469 knockdown suppressed the expression of genes associated with the ECM and collagen biosynthesis, indicating that ZNF469 plays a role in ECM regulation. Analysis Molecular medicine reports303https://www.ebi.ac.uk/biostudies/studies/S-EPMC12457930biostudies-literatureZNF469 promotes extracellular matrix production in normal and keloid dermal fibroblasts.PMC12457930Nokkeaw ABoonto TCharoenthanakitkul DAriyachet CfalseZNF469 promotes extracellular matrix production in normal and keloid dermal fibroblasts.Aberrant extracellular matrix (ECM) production by dermal fibroblasts drives fibrotic skin diseases, which has an adverse impact on the lives of patients. Current treatments are limited; therefore, the development of new antifibrotic strategies is necessary. The aim of the present study was to investigate zinc finger 469 (ZNF469) as a potential ECM regulator in skin fibrosis. ZNF469 was knocked down in dermal fibroblasts using doxycycline‑induced short hairpin RNA. ZNF469 knockdown was found to impair proliferation, migration, contraction and collagen production in dermal fibroblasts. In addition, RNA sequencing revealed that ZNF469 knockdown suppressed the expression of genes associated with the ECM and collagen biosynthesis, indicating that ZNF469 plays a role in ECM regulation. Analysis 2025-01-01T00:00:00Z2025 Nov2026-06-03T19:35:32.14Z2026-04-30T03:12:18.006ZS-EPMC124579304091021710.3892/mmr.2025.13668