{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ghignoli S"],"funding":["European Research Council"],"pagination":["e70019"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12457985"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(7-9)"],"pubmed_abstract":["The secretion of insulin and glucagon by pancreatic β and α cells, respectively, is critical for glucose homeostasis. While the insulin granule dynamics are well-characterized, the intracellular behavior of glucagon secretory granules (GSG) remains poorly understood. Here, we analyze the mobility of GSGs in αTC1-9 cells and insulin secretory granules (ISG) in INS-1E cells using spatiotemporal correlation spectroscopy and single-particle tracking (SPT), with a focus on the role of the cytoskeleton in regulating their transport. Under basal conditions, SPT classification reveals that GSGs predominantly exhibit diffusive motion (57.6% ± 10%), with smaller fractions categorized as almost immobile (35.8% ± 10.6%) or drifted (6.6% ± 3%), closely resembling ISG dynamics. By disrupting microtubule"],"journal":["Traffic (Copenhagen, Denmark)"],"pubmed_title":["Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics."],"pmcid":["PMC12457985"],"funding_grant_id":["866127"],"pubmed_authors":["Marchetti P","Luin S","Pugliese LA","Cardarelli F","Ferri G","Tesi M","Ghignoli S","De Lorenzi V"],"additional_accession":[]},"is_claimable":false,"name":"Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.","description":"The secretion of insulin and glucagon by pancreatic β and α cells, respectively, is critical for glucose homeostasis. While the insulin granule dynamics are well-characterized, the intracellular behavior of glucagon secretory granules (GSG) remains poorly understood. Here, we analyze the mobility of GSGs in αTC1-9 cells and insulin secretory granules (ISG) in INS-1E cells using spatiotemporal correlation spectroscopy and single-particle tracking (SPT), with a focus on the role of the cytoskeleton in regulating their transport. Under basal conditions, SPT classification reveals that GSGs predominantly exhibit diffusive motion (57.6% ± 10%), with smaller fractions categorized as almost immobile (35.8% ± 10.6%) or drifted (6.6% ± 3%), closely resembling ISG dynamics. By disrupting microtubule","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jul-Sep","modification":"2026-06-03T19:49:09.533Z","creation":"2026-05-01T03:10:34.653Z"},"accession":"S-EPMC12457985","cross_references":{"pubmed":["40988617"],"doi":["10.1111/tra.70019"]}}