biostudies-literatureGhignoli SEuropean Research Councile70019https://www.ebi.ac.uk/biostudies/studies/S-EPMC12457985biostudies-literatureUnknown26(7-9)The secretion of insulin and glucagon by pancreatic β and α cells, respectively, is critical for glucose homeostasis. While the insulin granule dynamics are well-characterized, the intracellular behavior of glucagon secretory granules (GSG) remains poorly understood. Here, we analyze the mobility of GSGs in αTC1-9 cells and insulin secretory granules (ISG) in INS-1E cells using spatiotemporal correlation spectroscopy and single-particle tracking (SPT), with a focus on the role of the cytoskeleton in regulating their transport. Under basal conditions, SPT classification reveals that GSGs predominantly exhibit diffusive motion (57.6% ± 10%), with smaller fractions categorized as almost immobile (35.8% ± 10.6%) or drifted (6.6% ± 3%), closely resembling ISG dynamics. By disrupting microtubuleTraffic (Copenhagen, Denmark)Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.PMC12457985866127Marchetti PLuin SPugliese LACardarelli FFerri GTesi MGhignoli SDe Lorenzi VfalseSpatiotemporal Analysis of Glucagon Secretory Granule Dynamics.The secretion of insulin and glucagon by pancreatic β and α cells, respectively, is critical for glucose homeostasis. While the insulin granule dynamics are well-characterized, the intracellular behavior of glucagon secretory granules (GSG) remains poorly understood. Here, we analyze the mobility of GSGs in αTC1-9 cells and insulin secretory granules (ISG) in INS-1E cells using spatiotemporal correlation spectroscopy and single-particle tracking (SPT), with a focus on the role of the cytoskeleton in regulating their transport. Under basal conditions, SPT classification reveals that GSGs predominantly exhibit diffusive motion (57.6% ± 10%), with smaller fractions categorized as almost immobile (35.8% ± 10.6%) or drifted (6.6% ± 3%), closely resembling ISG dynamics. By disrupting microtubule2025-01-01T00:00:00Z2025 Jul-Sep2026-06-03T19:49:09.533Z2026-05-01T03:10:34.653ZS-EPMC124579854098861710.1111/tra.70019