{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Hoang P"],"funding":["NICHD NIH HHS","NIDDK NIH HHS","NHLBI NIH HHS"],"pubmed_abstract":["The human heart, originating from the splanchnic mesoderm, is the first functional organ to develop, co-evolving with the foregut endoderm through reciprocal signaling. Previously, cardioid models offered new insights on cardiovascular cell lineages and tissue morphogenesis during heart development, while mesoderm-endoderm crosstalk remain incompletely understood. Here, we integrated micropatterned cardioids, CRISPR-engineered reporter hiPSCs, deep-tissue imaging, and single-cell RNA sequencing (scRNA-seq) to explore synergistic mesoderm-endoderm co-development. scRNA-seq with PHATE trajectory mapping reconstructed lineage bifurcations of mesoderm-heart and endoderm-foregut lineages, identifying key cell types in cardiac and hepatic development. Ligand-receptor interaction analysis highlig"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.09.17.676874"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12458260"],"repository":["biostudies-literature"],"pubmed_title":["Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids."],"pmcid":["PMC12458260"],"funding_grant_id":["R56 DK133147","R15 HD108720","R01 HL175050","R01 HD101130"],"pubmed_authors":["Yang H","Chai M","Zheng Y","Lian XL","Ma Z","De Vlaminck I","Cosgrove BD","Hoang P","McKellar DW","Mai NY","Tucker N","Amack J","Kowalczewski A"],"additional_accession":[]},"is_claimable":false,"name":"Size-Modulated Mesoderm-Endoderm Divergence and Myocardial Cavitation in Micropatterned Cardioids.","description":"The human heart, originating from the splanchnic mesoderm, is the first functional organ to develop, co-evolving with the foregut endoderm through reciprocal signaling. Previously, cardioid models offered new insights on cardiovascular cell lineages and tissue morphogenesis during heart development, while mesoderm-endoderm crosstalk remain incompletely understood. Here, we integrated micropatterned cardioids, CRISPR-engineered reporter hiPSCs, deep-tissue imaging, and single-cell RNA sequencing (scRNA-seq) to explore synergistic mesoderm-endoderm co-development. scRNA-seq with PHATE trajectory mapping reconstructed lineage bifurcations of mesoderm-heart and endoderm-foregut lineages, identifying key cell types in cardiac and hepatic development. Ligand-receptor interaction analysis highlig","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-03T03:11:06.669Z","creation":"2026-05-03T03:10:09.167Z"},"accession":"S-EPMC12458260","cross_references":{"pubmed":["41000917"],"doi":["10.1101/2025.09.17.676874"]}}