{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Krahn AH"],"funding":["NCI NIH HHS","National Institutes of Health","NIGMS NIH HHS"],"pubmed_abstract":["The traffic pathways that recycle resident Golgi proteins during cisternal maturation are not completely defined. We addressed this challenge using the yeast <i>Saccharomyces cerevisiae</i>, in which maturation of individual cisternae can be visualized directly. A new assay captures a specific population of Golgi-derived vesicles at the bud neck, thereby revealing which resident Golgi proteins are carried as cargo in those vesicles. This method supplies evidence for at least three classes of intra-Golgi vesicles with different cargo compositions. Consistent with our previously published data, one class of vesicles mediates a late pathway of intra-Golgi recycling with the aid of the AP-1 and Ent5 clathrin adaptors, and a second class of vesicles mediates an early pathway of intra-Golgi recycling with the aid of the COPI vesicle coat. Here, we identify another COPI-dependent pathway of intra-Golgi recycling and show that it operates kinetically between the two previously known pathways. Thus, intra-Golgi recycling is mediated by multiple COPI-dependent pathways followed by a clathrin-dependent pathway."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.09.20.677526"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12458367"],"repository":["biostudies-literature"],"pubmed_title":["COPI-dependent intra-Golgi recycling at an intermediate stage of cisternal maturation."],"pmcid":["PMC12458367"],"funding_grant_id":["T32 GM007183","R35 GM144050","P30 CA014599"],"pubmed_authors":["Glick BS","Lee-Smith CF","Pantazopoulou A","Austin J","Johnson N","Krahn AH"],"additional_accession":[]},"is_claimable":false,"name":"COPI-dependent intra-Golgi recycling at an intermediate stage of cisternal maturation.","description":"The traffic pathways that recycle resident Golgi proteins during cisternal maturation are not completely defined. We addressed this challenge using the yeast <i>Saccharomyces cerevisiae</i>, in which maturation of individual cisternae can be visualized directly. A new assay captures a specific population of Golgi-derived vesicles at the bud neck, thereby revealing which resident Golgi proteins are carried as cargo in those vesicles. This method supplies evidence for at least three classes of intra-Golgi vesicles with different cargo compositions. Consistent with our previously published data, one class of vesicles mediates a late pathway of intra-Golgi recycling with the aid of the AP-1 and Ent5 clathrin adaptors, and a second class of vesicles mediates an early pathway of intra-Golgi recycling with the aid of the COPI vesicle coat. Here, we identify another COPI-dependent pathway of intra-Golgi recycling and show that it operates kinetically between the two previously known pathways. Thus, intra-Golgi recycling is mediated by multiple COPI-dependent pathways followed by a clathrin-dependent pathway.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-04T03:21:01.422Z","creation":"2026-05-04T03:14:25.638Z"},"accession":"S-EPMC12458367","cross_references":{"pubmed":["41000804"],"doi":["10.1101/2025.09.20.677526"]}}