{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Roche B"],"funding":["NIGMS NIH HHS"],"pubmed_abstract":["In nature, most cells exist in a quiescent G<sub>0</sub> state in which cellular homeostasis must be rigorously maintained in the absence of cell division. Non-coding RNAs are prevalent in G<sub>0</sub> and are important regulators of development and differentiation, but their function in quiescence is unclear. Here, we identify pre-rRNA as a direct target of the RNase III enzyme Dicer specifically in quiescence. Dicer is physically present at the rDNA, and improper rRNA processing in mutants results in a nucleolar stress response involving a novel <i>trans</i>-acting non-coding RNA (RiboCop) in complex with the highly conserved proteins Enp2/NOL10 and RNase H1. RiboCop is complementary to unprocessed pre-rRNA and triggers rDNA repeat silencing via Sir2, RENT, and histone H3-lysine-9 (H3K9) methylation. Thus RiboCop silences rDNA specifically during dormancy, when silencing of non-functional rRNA becomes essential."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.09.16.676644"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12458480"],"repository":["biostudies-literature"],"pubmed_title":["RiboCop surveils pre-rRNA processing by Dicer in cellular quiescence."],"pmcid":["PMC12458480"],"funding_grant_id":["R35 GM144206","P20 GM104360"],"pubmed_authors":["Roche B","Martienssen RA"],"additional_accession":[]},"is_claimable":false,"name":"RiboCop surveils pre-rRNA processing by Dicer in cellular quiescence.","description":"In nature, most cells exist in a quiescent G<sub>0</sub> state in which cellular homeostasis must be rigorously maintained in the absence of cell division. Non-coding RNAs are prevalent in G<sub>0</sub> and are important regulators of development and differentiation, but their function in quiescence is unclear. Here, we identify pre-rRNA as a direct target of the RNase III enzyme Dicer specifically in quiescence. Dicer is physically present at the rDNA, and improper rRNA processing in mutants results in a nucleolar stress response involving a novel <i>trans</i>-acting non-coding RNA (RiboCop) in complex with the highly conserved proteins Enp2/NOL10 and RNase H1. RiboCop is complementary to unprocessed pre-rRNA and triggers rDNA repeat silencing via Sir2, RENT, and histone H3-lysine-9 (H3K9) methylation. Thus RiboCop silences rDNA specifically during dormancy, when silencing of non-functional rRNA becomes essential.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-04T03:16:14.608Z","creation":"2026-05-04T03:13:29.78Z"},"accession":"S-EPMC12458480","cross_references":{"pubmed":["41000809"],"doi":["10.1101/2025.09.16.676644"]}}