{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Shuken SR"],"funding":["NIA NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["The GoDig platform enables sensitive, multiplexed targeted pathway proteomics without manual scheduling or synthetic standards. Here we present GoDig 2.0, which increases sample multiplexing to 35-fold, improves time efficiency and reduces scan delays for higher success rates, and allows flexible spectral and elution library generation from different mass spectrometry data types. GoDig 2.0 measures 2.4× more targets than GoDig 1.0, quantifying >99% of 800 peptides in a single run. We compiled a library of 23,989 human phosphorylation sites from a phosphoproteomic dataset and used it to profile kinase signaling differences across cell lines. In human brain tissue, we established a hyperphosphorylated tau assay including pTau127, revealing potential biomarkers for Alzheimer's disease. We als"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.09.10.675380"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12458933"],"repository":["biostudies-literature"],"pubmed_title":["Next-Generation Multiplexed Targeted Proteomics Quantifies Post-Translational Modifications, Compound-Protein Interactions, and Disease Biomarkers with High Throughput."],"pmcid":["PMC12458933"],"funding_grant_id":["R01 GM132129","K22 CA282268","K99 AG088297","R01 GM067945"],"pubmed_authors":["Shuken SR","Dawson SL","Gassaway BM","Beard CR","Paulo JA","Yu Q","Frere GA","Gygi SP","Canterbury JD","Zuniga NR","McNerney MW","Ooi KH"],"additional_accession":[]},"is_claimable":false,"name":"Next-Generation Multiplexed Targeted Proteomics Quantifies Post-Translational Modifications, Compound-Protein Interactions, and Disease Biomarkers with High Throughput.","description":"The GoDig platform enables sensitive, multiplexed targeted pathway proteomics without manual scheduling or synthetic standards. Here we present GoDig 2.0, which increases sample multiplexing to 35-fold, improves time efficiency and reduces scan delays for higher success rates, and allows flexible spectral and elution library generation from different mass spectrometry data types. GoDig 2.0 measures 2.4× more targets than GoDig 1.0, quantifying >99% of 800 peptides in a single run. We compiled a library of 23,989 human phosphorylation sites from a phosphoproteomic dataset and used it to profile kinase signaling differences across cell lines. In human brain tissue, we established a hyperphosphorylated tau assay including pTau127, revealing potential biomarkers for Alzheimer's disease. We als","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-30T03:23:17.411Z","creation":"2026-06-30T03:16:12.234Z"},"accession":"S-EPMC12458933","cross_references":{"pubmed":["41000625"],"doi":["10.1101/2025.09.10.675380"]}}