{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Shuken SR"],"funding":["NIA NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Existing targeted mass spectrometry methods require synthetic standard peptides, manual scan scheduling, or data libraries to quantify target proteins. Here we present GoDig-LiF, which uses spectra and retention times predicted by the Prosit-TMT model in place of data libraries, enabling targeted proteomics with only a tandem mass tag-labeled sample, target list, and mass spectrometer. We applied GoDig-LiF to the quantification of mutated proteins in cancer cell lines, including KRAS G13D."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.09.10.675407"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12458936"],"repository":["biostudies-literature"],"pubmed_title":["Library-Free Multiplexed Targeted Proteomics Enables Quantitative Protein-Level Genotyping."],"pmcid":["PMC12458936"],"funding_grant_id":["K99 AG088297","R01 GM067945"],"pubmed_authors":["Shuken SR","Gygi SP"],"additional_accession":[]},"is_claimable":false,"name":"Library-Free Multiplexed Targeted Proteomics Enables Quantitative Protein-Level Genotyping.","description":"Existing targeted mass spectrometry methods require synthetic standard peptides, manual scan scheduling, or data libraries to quantify target proteins. Here we present GoDig-LiF, which uses spectra and retention times predicted by the Prosit-TMT model in place of data libraries, enabling targeted proteomics with only a tandem mass tag-labeled sample, target list, and mass spectrometer. We applied GoDig-LiF to the quantification of mutated proteins in cancer cell lines, including KRAS G13D.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-04T03:20:57.382Z","creation":"2026-05-04T03:14:25.384Z"},"accession":"S-EPMC12458936","cross_references":{"pubmed":["41001004"],"doi":["10.1101/2025.09.10.675407"]}}