{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Li H"],"funding":["NCI NIH HHS"],"pubmed_abstract":["Most advanced melanomas initially respond to targeted therapy but eventually relapse. Rather than acquiring new mutations, resistance is driven by drug-tolerant persister cells that enter a reversible drug-refractory state. We developed MeRLin, a high-resolution lineage tracing platform integrating cellular barcoding, single-cell transcriptomics, RNA fluorescence in situ hybridization (FISH), and computational analyses to track clonal and transcriptional dynamics in patient-derived melanoma models during prolonged therapy. Clonal dynamics revealed that persister subpopulations first responded to treatment but persisted and expanded during minimal residual disease, ultimately leading to tumor recurrence. Pre-treatment melanoma populations diversified into four conserved persister states cha"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.09.16.676608"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12458950"],"repository":["biostudies-literature"],"pubmed_title":["Clonal dynamics shaped by diverse drug-tolerant persister states in melanoma resistance."],"pmcid":["PMC12458950"],"funding_grant_id":["P50 CA261608","R01 CA240362","U54 CA224070","P01 CA114046","R01 CA238237","R01 CA241148"],"pubmed_authors":["Fang H","Hu T","Herlyn M","Bin T","Patterson A","Jiang S","Brown GS","Wei Z","Wang L","Madzo J","Hoon DS","Dunne M","Thomas M","Ren J","Salazar ER","Chen Y","Promi N","Li H","Fingerman D","Auslander N","Thacker G","Villanueva J","Kaster J","Zheng Q","Mou H","Li L","Raj A","Xiao M","Reale M","Gao L","Zhang X","Zhu X","Qi C"],"additional_accession":[]},"is_claimable":false,"name":"Clonal dynamics shaped by diverse drug-tolerant persister states in melanoma resistance.","description":"Most advanced melanomas initially respond to targeted therapy but eventually relapse. Rather than acquiring new mutations, resistance is driven by drug-tolerant persister cells that enter a reversible drug-refractory state. We developed MeRLin, a high-resolution lineage tracing platform integrating cellular barcoding, single-cell transcriptomics, RNA fluorescence in situ hybridization (FISH), and computational analyses to track clonal and transcriptional dynamics in patient-derived melanoma models during prolonged therapy. Clonal dynamics revealed that persister subpopulations first responded to treatment but persisted and expanded during minimal residual disease, ultimately leading to tumor recurrence. Pre-treatment melanoma populations diversified into four conserved persister states cha","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-04T03:14:35.348Z","creation":"2026-05-04T03:13:05.319Z"},"accession":"S-EPMC12458950","cross_references":{"pubmed":["41000875"],"doi":["10.1101/2025.09.16.676608"]}}