{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Weingarten-Gabbay S"],"funding":["Howard Hughes Medical Institute","NIAID NIH HHS","NCI NIH HHS"],"pagination":["1218-1224"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12459998"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["388(6752)"],"pubmed_abstract":["Defining viral proteomes is crucial to understanding viral life cycles and immune recognition but the landscape of translated regions remains unknown for most viruses. We have developed massively parallel ribosome profiling (MPRP) to determine open reading frames (ORFs) across tens of thousands of designed oligonucleotides. MPRP identified 4208 unannotated ORFs in 679 human-associated viral genomes. We found viral peptides originating from detected noncanonical ORFs presented on class-I human leukocyte antigen in infected cells and hundreds of upstream ORFs that likely modulate translation initiation of viral proteins. The discovery of viral ORFs across a wide range of viral families-including highly pathogenic viruses-expands the repertoire of vaccine targets and reveals potential cis-reg"],"journal":["Science (New York, N.Y.)"],"pubmed_title":["Pan-viral ORFs discovery using massively parallel ribosome profiling."],"pmcid":["PMC12459998"],"funding_grant_id":["U24 CA270823","R01 CA057973","P01 CA206978","U01 CA271402","U19 AI110818"],"pubmed_authors":["Clauser KR","Boehm CK","Stanton AC","Verzani EK","Freije CA","Welch NL","Carr SA","Bauer MR","Lopez D","Weingarten-Gabbay S","Hensley LE","Rice CM","Sabeti PC","Klaeger S","Abelin JG","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Pan-viral ORFs discovery using massively parallel ribosome profiling.","description":"Defining viral proteomes is crucial to understanding viral life cycles and immune recognition but the landscape of translated regions remains unknown for most viruses. We have developed massively parallel ribosome profiling (MPRP) to determine open reading frames (ORFs) across tens of thousands of designed oligonucleotides. MPRP identified 4208 unannotated ORFs in 679 human-associated viral genomes. We found viral peptides originating from detected noncanonical ORFs presented on class-I human leukocyte antigen in infected cells and hundreds of upstream ORFs that likely modulate translation initiation of viral proteins. The discovery of viral ORFs across a wide range of viral families-including highly pathogenic viruses-expands the repertoire of vaccine targets and reveals potential cis-reg","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-06-03T19:47:46.981Z","creation":"2026-05-01T03:10:28.094Z"},"accession":"S-EPMC12459998","cross_references":{"pubmed":["40504907"],"doi":["10.1126/science.ado6670"]}}