{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["645(8082)"],"submitter":["Gavrilov AA"],"pubmed_abstract":["Unravelling how genomes are spatially organized and how their three-dimensional (3D) architecture drives cellular functions remains a major challenge in biology<sup>1,2</sup>. In bacteria, genomic DNA is compacted into a highly ordered, condensed state called nucleoid<sup>3-5</sup>. Despite progress in characterizing bacterial 3D genome architecture over recent decades<sup>6-8</sup>, the fine structure and functional organization of the nucleoid remain elusive due to low-resolution contact maps from methods such as Hi-C<sup>9-11</sup>. Here we developed an enhanced Micro-C chromosome conformation capture, achieving 10-base pair (bp) resolution. This ultra-high-resolution analysis reveals elemental spatial structures in the Escherichia coli nucleoid, including chromosomal hairpins (CHINs) a"],"journal":["Nature"],"pagination":["1060-1070"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12460168"],"repository":["biostudies-literature"],"pubmed_title":["Elementary 3D organization of active and silenced E. coli genome."],"pmcid":["PMC12460168"],"pubmed_authors":["Shamovsky I","Blavatnik A","Proshkin S","Lahiri S","Rasouly A","Shamovsky Y","Epshtein V","Evko G","Razin SV","Zhegalova I","Nudler E","Gavrilov AA","Rothenberg E"],"additional_accession":[]},"is_claimable":false,"name":"Elementary 3D organization of active and silenced E. coli genome.","description":"Unravelling how genomes are spatially organized and how their three-dimensional (3D) architecture drives cellular functions remains a major challenge in biology<sup>1,2</sup>. In bacteria, genomic DNA is compacted into a highly ordered, condensed state called nucleoid<sup>3-5</sup>. Despite progress in characterizing bacterial 3D genome architecture over recent decades<sup>6-8</sup>, the fine structure and functional organization of the nucleoid remain elusive due to low-resolution contact maps from methods such as Hi-C<sup>9-11</sup>. Here we developed an enhanced Micro-C chromosome conformation capture, achieving 10-base pair (bp) resolution. This ultra-high-resolution analysis reveals elemental spatial structures in the Escherichia coli nucleoid, including chromosomal hairpins (CHINs) a","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-07-15T06:45:40.511Z","creation":"2026-06-30T03:22:39.678Z"},"accession":"S-EPMC12460168","cross_references":{"pubmed":["40804527"],"doi":["10.1038/s41586-025-09396-y"]}}