biostudies-literatureMulamba CEuropean & Developing Countries Clinical Trials Partnership (EDCTP)1589061https://www.ebi.ac.uk/biostudies/studies/S-EPMC12460233biostudies-literatureUnknown16Transmission-blocking vaccines are among the novel tools under development for malaria control and elimination. Understanding the human immune response to the sexual stages of <i>Plasmodium falciparum</i> is essential for progressing transmission-blocking vaccine development. A serosurvey was conducted in Tanzania, from May to August 2022 among 290 participants, consisting of 114 children (5-12 years), 44 adolescents (13-17 years), and 132 adults (18-45 years). The participants were tested for malaria parasites using quantitative polymerase chain reaction, and standardized enzyme-linked immunosorbent assays were performed to detect the presence of IgG antibodies against transmission-blocking target antigens-Pfs230D1M, Pfs48/45, and Pfs25. A set of 10 plasma samples that were reactive to PfFrontiers in immunologySeroprevalence of antibodies to &lt;i&gt;Plasmodium falciparum&lt;/i&gt; transmission-blocking target proteins Pfs230D1M and Pfs48/45 in Tanzanian populations of diverse malaria transmission intensity.PMC12460233RIA2016V-1649Mulamba CMiura KLong CAMtaka IKamage JWilliams CLazaro LOKreppel KMekhaiel DNkumama IOdufuwa OGOlotu AIKalinga WFfalseSeroprevalence of antibodies to &lt;i&gt;Plasmodium falciparum&lt;/i&gt; transmission-blocking target proteins Pfs230D1M and Pfs48/45 in Tanzanian populations of diverse malaria transmission intensity.Transmission-blocking vaccines are among the novel tools under development for malaria control and elimination. Understanding the human immune response to the sexual stages of <i>Plasmodium falciparum</i> is essential for progressing transmission-blocking vaccine development. A serosurvey was conducted in Tanzania, from May to August 2022 among 290 participants, consisting of 114 children (5-12 years), 44 adolescents (13-17 years), and 132 adults (18-45 years). The participants were tested for malaria parasites using quantitative polymerase chain reaction, and standardized enzyme-linked immunosorbent assays were performed to detect the presence of IgG antibodies against transmission-blocking target antigens-Pfs230D1M, Pfs48/45, and Pfs25. A set of 10 plasma samples that were reactive to Pf2025-01-01T00:00:00Z20252026-06-03T21:40:40.891Z2026-05-02T03:08:08.433ZS-EPMC124602334101904810.3389/fimmu.2025.1589061