{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang J"],"funding":["NHLBI NIH HHS","U.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI)"],"pagination":["8363"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12460811"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["During development, myocardial contractile force and intracardiac hemodynamic shear stress coordinate the initiation of trabeculation. While Snail family genes are well-recognized transcription factors of epithelial-to-mesenchymal transition, snai1b-positive cardiomyocytes are sparsely distributed in the ventricle of zebrafish at 4 days post-fertilization. Isoproterenol treatment significantly increases the number of snai1b-positive cardiomyocytes, of which 80% are Notch-negative. CRISPR-activation of snai1b leads to 51.6% cardiomyocytes forming trabeculae, whereas CRISPR-repression reduces trabecular cardiomyocytes to 6.7% under isoproterenol. In addition, 36.7% of snai1b-repressed cardiomyocytes undergo apical delamination. 4-D strain analysis demonstrates that isoproterenol increases th"],"journal":["Nature communications"],"pubmed_title":["Mechanically activated snai1b coordinates the initiation of myocardial delamination for trabeculation."],"pmcid":["PMC12460811"],"funding_grant_id":["R01HL159970","T32HL144449","R01 HL159970","R01 HL129727","R01 HL165318","T32 HL144449","R01HL165318","R01HL129727"],"pubmed_authors":["Liu J","O'Donnell R","Hsiai TK","Brown AL","Langenbacher A","Zhu E","Wang J","Chen JN","Marsden AL","Zheng CZ","Hsu JJ","Zhao P","Park SK","Yokota T"],"additional_accession":[]},"is_claimable":false,"name":"Mechanically activated snai1b coordinates the initiation of myocardial delamination for trabeculation.","description":"During development, myocardial contractile force and intracardiac hemodynamic shear stress coordinate the initiation of trabeculation. While Snail family genes are well-recognized transcription factors of epithelial-to-mesenchymal transition, snai1b-positive cardiomyocytes are sparsely distributed in the ventricle of zebrafish at 4 days post-fertilization. Isoproterenol treatment significantly increases the number of snai1b-positive cardiomyocytes, of which 80% are Notch-negative. CRISPR-activation of snai1b leads to 51.6% cardiomyocytes forming trabeculae, whereas CRISPR-repression reduces trabecular cardiomyocytes to 6.7% under isoproterenol. In addition, 36.7% of snai1b-repressed cardiomyocytes undergo apical delamination. 4-D strain analysis demonstrates that isoproterenol increases th","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-07-15T09:49:00.7Z","creation":"2026-07-02T03:12:09.877Z"},"accession":"S-EPMC12460811","cross_references":{"pubmed":["40993149"],"doi":["10.1038/s41467-025-62285-w"]}}