{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gockel J"],"funding":["Horizon 2020 Framework Programme","Leibniz Institute for Natural Product Research and Infection BiologyHans Knöll Institute","H2020 Marie Skłodowska-Curie Actions"],"pagination":["101110"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12461585"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(8)"],"pubmed_abstract":["Phenotypic variation between malaria parasites is a major contributor to the pathogen's success, facilitated by heritable yet dynamic changes in (hetero)chromatin structure. Currently, the chromatin landscape is mostly profiled by chromatin immunoprecipitation sequencing (ChIP-seq), which has several drawbacks: (1) GC-content-related artifacts, (2) substantial material requirement, and (3) a labor-intensive protocol. To overcome these limitations, we adapted cleavage under targets and tagmentation (CUT&Tag) to Plasmodium falciparum. Despite the AT richness of the genome, CUT&Tag results in reproducible heterochromatin profiles concordant with ChIP-seq data while using as little as 10,000 nuclei or crude parasite isolates. We also developed DiBioCUT&Tag, a method utilizing dimerization-indu"],"journal":["Cell reports methods"],"pubmed_title":["CUT&Tag and DiBioCUT&Tag enable investigation of the AT-rich epigenome of Plasmodium falciparum from low-input samples."],"pmcid":["PMC12461585"],"funding_grant_id":["K328/2020","860875"],"pubmed_authors":["Spielmann T","Bartfai R","Kimmel J","Ramon-Zamorano G","Gockel J"],"additional_accession":[]},"is_claimable":false,"name":"CUT&Tag and DiBioCUT&Tag enable investigation of the AT-rich epigenome of Plasmodium falciparum from low-input samples.","description":"Phenotypic variation between malaria parasites is a major contributor to the pathogen's success, facilitated by heritable yet dynamic changes in (hetero)chromatin structure. Currently, the chromatin landscape is mostly profiled by chromatin immunoprecipitation sequencing (ChIP-seq), which has several drawbacks: (1) GC-content-related artifacts, (2) substantial material requirement, and (3) a labor-intensive protocol. To overcome these limitations, we adapted cleavage under targets and tagmentation (CUT&Tag) to Plasmodium falciparum. Despite the AT richness of the genome, CUT&Tag results in reproducible heterochromatin profiles concordant with ChIP-seq data while using as little as 10,000 nuclei or crude parasite isolates. We also developed DiBioCUT&Tag, a method utilizing dimerization-indu","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-03T21:11:27.513Z","creation":"2026-05-01T03:11:08.468Z"},"accession":"S-EPMC12461585","cross_references":{"pubmed":["40675147"],"doi":["10.1016/j.crmeth.2025.101110"]}}