biostudies-literatureUnknown13(9)Wu JThis study was supported by Weilong Zhong (Nos. 2021A1515111009 and 82203900), Bo Yu (Nos. SZSM202311029 and SZXK040), and Xia Dou (Nos. JCYJ20210324105411030 and KYQD2021016).<h4>Background</h4>The available evidence increasingly indicates that SERPINB3/4 plays a pivotal role in the progression of inflammatory diseases and may serve as a valuable biomarker for atopic dermatitis and psoriasis. However, the expression of SERPINB3/4 in the serum of patients with moderate-to-severe prurigo nodularis (PN) remains poorly understood.<h4>Objective</h4>To find a promising biomarker for monitoring the severity and activity of moderate-to-severe PN patients.<h4>Methods</h4>A total of 41 patients with moderate-to-severe PN and 20 healthy subjects were included in the study. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum SERPINB3/4 expression. Cutaneous SERPINB3/4 expression was evaluated by immunohistochemistry (IHC). Serum SERPINB3/4 levels werImmunity, inflammation and diseasee70263https://www.ebi.ac.uk/biostudies/studies/S-EPMC12461664biostudies-literatureSerum SERPINB3/4 in Moderate-to-Severe Prurigo Nodularis: A Potential Biomarker for Disease Severity and Eosinophil-Related Parameters.PMC12461664Dou XWu JLiao YZhang KYu BShao YWu CGe JZhong WYang CfalseSerum SERPINB3/4 in Moderate-to-Severe Prurigo Nodularis: A Potential Biomarker for Disease Severity and Eosinophil-Related Parameters.<h4>Background</h4>The available evidence increasingly indicates that SERPINB3/4 plays a pivotal role in the progression of inflammatory diseases and may serve as a valuable biomarker for atopic dermatitis and psoriasis. However, the expression of SERPINB3/4 in the serum of patients with moderate-to-severe prurigo nodularis (PN) remains poorly understood.<h4>Objective</h4>To find a promising biomarker for monitoring the severity and activity of moderate-to-severe PN patients.<h4>Methods</h4>A total of 41 patients with moderate-to-severe PN and 20 healthy subjects were included in the study. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum SERPINB3/4 expression. Cutaneous SERPINB3/4 expression was evaluated by immunohistochemistry (IHC). Serum SERPINB3/4 levels wer2025-01-01T00:00:00Z2025 Sep2026-06-01T03:07:32.286Z2026-06-01T03:06:01.338ZS-EPMC124616644099589210.1002/iid3.70263