biostudies-literatureLuce AEpimetPNRR - MISSIONE 4 COMPONENTE 2 "Dalla Ricerca all'Impresa".e1008https://www.ebi.ac.uk/biostudies/studies/S-EPMC12462032biostudies-literatureUnknown23(1)<h4>Background</h4>Hepatocellular carcinoma (HCC) remains a significant clinical challenge due to limited diagnostic and therapeutic options. Non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), play key roles in cancer biology. Our previous findings showed that miR-423-5p enhances anti-cancer effects on HCC patients treated with sorafenib by promoting autophagy. Here, we investigated the molecular mechanisms underlying miR-423-5p function through a comprehensive proteomic approach.<h4>Methods</h4>We generated an HCC cell line stably overexpressing miR-423-5p via lentiviral transduction. Total proteins were extracted from SNU-387 cells, enzymatically digested into peptides, and subsequently analysed by liquid chromatography-tandem mass spectrometry (LC-MS/M). Raw spectral data were procesJournal of translational medicineProteomic profiling identifies miR-423-5p as a modulator of oncogenic metabolism in HCC.PMC12462032(PE00000019)(F/310034/03/X56)Luce ABoocock DJTathode MSCoveney CVerderio EAMCossu AMZappavigna SRomano MPMisso GVitale GSperlongano RCaraglia MDe Iesu MRSimeone IMele LBocchetti MfalseProteomic profiling identifies miR-423-5p as a modulator of oncogenic metabolism in HCC.<h4>Background</h4>Hepatocellular carcinoma (HCC) remains a significant clinical challenge due to limited diagnostic and therapeutic options. Non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), play key roles in cancer biology. Our previous findings showed that miR-423-5p enhances anti-cancer effects on HCC patients treated with sorafenib by promoting autophagy. Here, we investigated the molecular mechanisms underlying miR-423-5p function through a comprehensive proteomic approach.<h4>Methods</h4>We generated an HCC cell line stably overexpressing miR-423-5p via lentiviral transduction. Total proteins were extracted from SNU-387 cells, enzymatically digested into peptides, and subsequently analysed by liquid chromatography-tandem mass spectrometry (LC-MS/M). Raw spectral data were proces2025-01-01T00:00:00Z2025 Sep2026-07-15T06:48:46.653Z2026-06-30T03:23:27.538ZS-EPMC124620324099365710.1186/s12967-025-07039-4