{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Oorlynck L"],"funding":["Fonds Wetenschappelijk Onderzoek","Bijzonder Onderzoeksfonds UGent"],"pagination":["e2401985"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12464656"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(9)"],"pubmed_abstract":["An improved small-circle scanning fluorescence correlation spectroscopy (sFCS) technique is introduced by combining acousto-optical laser scanning with fitting the autocorrelation function in the frequency domain. The technique is validated using both simulation and experimental data on various fluorescent nanoparticles, including polystyrene beads, CdSe/CdS quantum dots, and lipid nanoparticles. Then, the sFCS method is used to investigate the adsorption of in-house synthesized poly(2-guanidinoethyl methacrylate) (PGUMA) polymers on polystyrene beads as a model system for polymer-coated particles in biomedical and gene delivery applications. Using the particle diffusion and illumination beam waist values obtained from our sFCS analysis, regions of polymer concentrations are identified where polymer-particle complexes remain stable. An increase in hydrodynamic size is also observed with the molecular mass of the adsorbed polymer. Beyond quantifying polymer-particle stability and hydrodynamic size, the sFCS technique offers the advantage of not requiring a time-consuming calibration step for the measurement volume, unlike standard FCS."],"journal":["Small methods"],"pubmed_title":["Cationic Polyelectrolyte Adsorption onto Anionic Nanoparticles Analyzed with Frequency-Domain Scanning Fluorescence Correlation Spectroscopy."],"pmcid":["PMC12464656"],"funding_grant_id":["1SD0721N","1SA2720N","G0H7520N","I006920N","BOF.BAS.2022.0023.01","G037221N"],"pubmed_authors":["Remaut K","Van Daele L","Sumit S","Ussembayev YY","Oorlynck L","Moreels I","Dubruel P","Myslovska A","Goddaer S","Strubbe F"],"additional_accession":[]},"is_claimable":false,"name":"Cationic Polyelectrolyte Adsorption onto Anionic Nanoparticles Analyzed with Frequency-Domain Scanning Fluorescence Correlation Spectroscopy.","description":"An improved small-circle scanning fluorescence correlation spectroscopy (sFCS) technique is introduced by combining acousto-optical laser scanning with fitting the autocorrelation function in the frequency domain. The technique is validated using both simulation and experimental data on various fluorescent nanoparticles, including polystyrene beads, CdSe/CdS quantum dots, and lipid nanoparticles. Then, the sFCS method is used to investigate the adsorption of in-house synthesized poly(2-guanidinoethyl methacrylate) (PGUMA) polymers on polystyrene beads as a model system for polymer-coated particles in biomedical and gene delivery applications. Using the particle diffusion and illumination beam waist values obtained from our sFCS analysis, regions of polymer concentrations are identified where polymer-particle complexes remain stable. An increase in hydrodynamic size is also observed with the molecular mass of the adsorbed polymer. Beyond quantifying polymer-particle stability and hydrodynamic size, the sFCS technique offers the advantage of not requiring a time-consuming calibration step for the measurement volume, unlike standard FCS.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T21:08:14.59Z","creation":"2026-05-01T03:10:47.4Z"},"accession":"S-EPMC12464656","cross_references":{"pubmed":["40211559"],"doi":["10.1002/smtd.202401985"]}}