biostudies-literatureZhang TNatural Science Foundation of Hunan ProvinceNational Natural Science Foundation of ChinaUniversity of South Chinagkaf972https://www.ebi.ac.uk/biostudies/studies/S-EPMC12464824biostudies-literatureUnknown53(18)Alternative splicing (AS) regulates the diversity and level of the proteome. The specificity in AS is in turn regulated by RNA-binding proteins, but our understanding of how they act is far from complete. Here, we identify the Angel wing (Anw) protein, previously CG10948, as a novel AS regulator. Loss of Anw in Drosophila disrupts splicing in muscle genes and subsequently muscle function. Based on a mini-gene assay in which Anw and its RNA targets are co-expressed in cultured cells, we demonstrated orthologous splicing regulation of the mini-gene transcripts, interaction between Anw and its RNA targets, and a remarkable functional conservation among Anw homologs. Anw forms nuclear foci, and genetic ablation of Anw domains suggests that maintaining distinctive features of these foci is impoNucleic acids researchAngel wing: a class of alternative splicing regulators in animals.PMC1246482432211013282023JJ40536Cui MRong YSZhang TTan XXiao YLi KfalseAngel wing: a class of alternative splicing regulators in animals.Alternative splicing (AS) regulates the diversity and level of the proteome. The specificity in AS is in turn regulated by RNA-binding proteins, but our understanding of how they act is far from complete. Here, we identify the Angel wing (Anw) protein, previously CG10948, as a novel AS regulator. Loss of Anw in Drosophila disrupts splicing in muscle genes and subsequently muscle function. Based on a mini-gene assay in which Anw and its RNA targets are co-expressed in cultured cells, we demonstrated orthologous splicing regulation of the mini-gene transcripts, interaction between Anw and its RNA targets, and a remarkable functional conservation among Anw homologs. Anw forms nuclear foci, and genetic ablation of Anw domains suggests that maintaining distinctive features of these foci is impo2025-01-01T00:00:00Z2025 Sep2026-06-30T03:33:10.41Z2026-06-30T03:28:23.305ZS-EPMC124648244100202710.1093/nar/gkaf972