{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["van Schaik T"],"funding":["Deutsche Forschungsgemeinschaft","European Union","Medical Research Council","Dutch Research Council (NWO)","Dutch Cancer Society","EU-MUR MSCA Young Researcher"],"pagination":["gkaf964"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12464825"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(18)"],"pubmed_abstract":["Lamina-associated domains (LADs) are megabase-sized genomic regions anchored to the nuclear lamina (NL). Factors controlling the interactions of the genome with the NL have largely remained elusive. Here, we identified DNA topoisomerase 2 beta (TOP2B) as a regulator of these interactions. TOP2B binds predominantly to inter-LAD (iLAD) chromatin and its depletion results in a partial loss of genomic partitioning between LADs and iLADs, suggesting that this enzyme might protect specific iLADs from interacting with the NL. TOP2B depletion affects LAD interactions with lamin B receptor (LBR) more than with lamins. LBR depletion phenocopies the effects of TOP2B depletion, despite the different positioning of the two proteins in the genome. This suggests a complementary mechanism for organizing t"],"journal":["Nucleic acids research"],"pubmed_title":["Coordinated control of genome-nuclear lamina interactions by topoisomerase 2B and lamin B receptor."],"pmcid":["PMC12464825"],"funding_grant_id":["838555","865459","694466","455784893","402733153-SPP 2202","58588","800924","MR/J00913X/1","MSCA_000020","393547839SFB 1361","MC_UU_00007/13"],"pubmed_authors":["Medema RH","de Wit E","Gilbert N","de Haas M","van Schaik T","van Steensel B","Manjon AG","Manzo SG","Magnitov M","Gothe HJ","Buckle AJ","Naughton C","Breda J","Roukos V"],"additional_accession":[]},"is_claimable":false,"name":"Coordinated control of genome-nuclear lamina interactions by topoisomerase 2B and lamin B receptor.","description":"Lamina-associated domains (LADs) are megabase-sized genomic regions anchored to the nuclear lamina (NL). Factors controlling the interactions of the genome with the NL have largely remained elusive. Here, we identified DNA topoisomerase 2 beta (TOP2B) as a regulator of these interactions. TOP2B binds predominantly to inter-LAD (iLAD) chromatin and its depletion results in a partial loss of genomic partitioning between LADs and iLADs, suggesting that this enzyme might protect specific iLADs from interacting with the NL. TOP2B depletion affects LAD interactions with lamin B receptor (LBR) more than with lamins. LBR depletion phenocopies the effects of TOP2B depletion, despite the different positioning of the two proteins in the genome. This suggests a complementary mechanism for organizing t","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T21:10:12.835Z","creation":"2026-05-01T03:11:00.21Z"},"accession":"S-EPMC12464825","cross_references":{"pubmed":["41002030"],"doi":["10.1093/nar/gkaf964"]}}