{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li K"],"funding":["Fundamental Research Funds for the Central Universities","Research Project of China Baoyuan Investment Co., Ltd","CAMS Innovation Fund for Medical Sciences","State Key Laboratory Special Fund","the Non-profit Central Research Institute Fund of the Chinese Academy of Medical Sciences","Wellcome Trust","Haihe Laboratory of Cell Ecosystem Innovation Fund"],"pagination":["1468-1479"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12464859"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(8)"],"pubmed_abstract":["<h4>Background</h4>The Cre/loxP system is most popular in mice, but its application in rats has largely lagged far behind. The rat is vital laboratory animal, especially in toxicological and neurological studies. Generating genetic tools to manipulate neurons in rats could benefit neurological research.<h4>Methods</h4>Using the CRISPR/Cas9 system, we inserted a Cre cassette into endogenous Thy1 and NeuN loci. Thy1-Cre rats featured a downstream P2A-linked insertion, while NeuN-Cre was inserted at the transcriptional start site. The Cre activity was assessed by crossing with a Cre reporter (Rosa26<sup>imCherry</sup>) rat and through analyzing mCherry expression patterns. The specificity of cell type was further confirmed by immunofluorescence with NeuN antibody. Phenotypic consequences were"],"journal":["Animal models and experimental medicine"],"pubmed_title":["Comparative analysis of two newly established Cre rat lines, NeuN-Cre and Thy1-Cre, for neurological research."],"pmcid":["PMC12464859"],"funding_grant_id":["2060204","2023-I2M-2-001","3332023164","HH24KYZX0007","2023-PT180-01.","Program CBYI202102","3332022040","202411","2021-I2M-1-024","2021-I2M-1-034"],"pubmed_authors":["Dong W","He J","Liu N","Guan F","Ma Y","Zhang L","Li K","Fan R","Qi X","Zhang X","Liu C","Wan G","Su Y","Gao S","Kong W"],"additional_accession":[]},"is_claimable":false,"name":"Comparative analysis of two newly established Cre rat lines, NeuN-Cre and Thy1-Cre, for neurological research.","description":"<h4>Background</h4>The Cre/loxP system is most popular in mice, but its application in rats has largely lagged far behind. The rat is vital laboratory animal, especially in toxicological and neurological studies. Generating genetic tools to manipulate neurons in rats could benefit neurological research.<h4>Methods</h4>Using the CRISPR/Cas9 system, we inserted a Cre cassette into endogenous Thy1 and NeuN loci. Thy1-Cre rats featured a downstream P2A-linked insertion, while NeuN-Cre was inserted at the transcriptional start site. The Cre activity was assessed by crossing with a Cre reporter (Rosa26<sup>imCherry</sup>) rat and through analyzing mCherry expression patterns. The specificity of cell type was further confirmed by immunofluorescence with NeuN antibody. Phenotypic consequences were","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-03T20:23:16.308Z","creation":"2026-05-01T03:10:08.669Z"},"accession":"S-EPMC12464859","cross_references":{"pubmed":["40557518"],"doi":["10.1002/ame2.70051"]}}