biostudies-literatureJin ZNational Natural Science Foundation of ChinaShanghai Scientific Research Project of ChinaNational Key Research and Development Program of China521https://www.ebi.ac.uk/biostudies/studies/S-EPMC12465172biostudies-literatureUnknown16(1)<h4>Introduction</h4>Osteosarcopenic fractures, an emerging geriatric syndrome characterized by sarcopenia-osteoporotic fractures coexistence, delayed fracture healing, and elevated risk of re-fracture. Limited research has investigated the mechanisms by which skeletal muscle satellite cells (SMSCs) promote muscle regeneration and osteoporotic fracture healing. The aim of this study was to investigate the impact of a traditional herbal decoction (HD), the Invigorate the Spleen and Tonify the Kidney Formula, on SMSC regulation, muscle regeneration, and fracture healing.<h4>Method</h4>Using conditional knockout mice, the role of SMSCs in promoting fracture healing and mitigating sarcopenia was evaluated by visualizing the fracture area and surrounding muscle tissue. The signaling pathways inStem cell research & therapyA traditional herbal decoction regulates skeletal muscle satellite cell osteogenesis and myogenesis for repairing osteosarcopenic fractures via β-catenin.PMC124651728197388320S219020002018YFC17043008230526281730107Li YDa WShi QWan HGao XTang DShen YXu HJin ZZhao YWu XfalseA traditional herbal decoction regulates skeletal muscle satellite cell osteogenesis and myogenesis for repairing osteosarcopenic fractures via β-catenin.<h4>Introduction</h4>Osteosarcopenic fractures, an emerging geriatric syndrome characterized by sarcopenia-osteoporotic fractures coexistence, delayed fracture healing, and elevated risk of re-fracture. Limited research has investigated the mechanisms by which skeletal muscle satellite cells (SMSCs) promote muscle regeneration and osteoporotic fracture healing. The aim of this study was to investigate the impact of a traditional herbal decoction (HD), the Invigorate the Spleen and Tonify the Kidney Formula, on SMSC regulation, muscle regeneration, and fracture healing.<h4>Method</h4>Using conditional knockout mice, the role of SMSCs in promoting fracture healing and mitigating sarcopenia was evaluated by visualizing the fracture area and surrounding muscle tissue. The signaling pathways in2025-01-01T00:00:00Z2025 Sep2026-06-03T21:48:54.359Z2026-05-02T03:10:57.466ZS-EPMC124651724101354210.1186/s13287-025-04642-6