{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bhuiyan SA"],"funding":["University of British Columbia"],"pagination":["842"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12465193"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(1)"],"pubmed_abstract":["<h4>Background</h4>The degree to which alternative RNA splicing influences the function and structure of voltage gated calcium channel (VGCC) splice variants is poorly understood. Here we used long-read RNA-sequencing to catalog rat Cacna1e (Cav2.3) splice variants, and computationally prioritize which are likely to impact channel function.<h4>Result</h4>We sequenced Cacna1e transcripts from rat thalamus using Oxford Nanopore sequencing yielding the structure of 2,110 Cacna1e splice variants. Of these, up to 154 had the potential encode for a functional channel based on predicted amino acid sequences. Our analysis revealed a total of 31 cassette splicing events (in various combinations) potentially affecting channel function, with three cassette exons appreciably expressed and conserved.<h"],"journal":["BMC genomics"],"pubmed_title":["Cataloging the potential functional diversity of Cacna1e splice variants using long-read sequencing."],"pmcid":["PMC12465193"],"funding_grant_id":["Michael Smith Laboratories Kickstart"],"pubmed_authors":["Pavlidis P","Snutch TP","Bhuiyan SA","Belmadani M","Tyson JR","Sicherman J"],"additional_accession":[]},"is_claimable":false,"name":"Cataloging the potential functional diversity of Cacna1e splice variants using long-read sequencing.","description":"<h4>Background</h4>The degree to which alternative RNA splicing influences the function and structure of voltage gated calcium channel (VGCC) splice variants is poorly understood. Here we used long-read RNA-sequencing to catalog rat Cacna1e (Cav2.3) splice variants, and computationally prioritize which are likely to impact channel function.<h4>Result</h4>We sequenced Cacna1e transcripts from rat thalamus using Oxford Nanopore sequencing yielding the structure of 2,110 Cacna1e splice variants. Of these, up to 154 had the potential encode for a functional channel based on predicted amino acid sequences. Our analysis revealed a total of 31 cassette splicing events (in various combinations) potentially affecting channel function, with three cassette exons appreciably expressed and conserved.<h","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T23:16:36.26Z","creation":"2026-05-03T03:10:11.324Z"},"accession":"S-EPMC12465193","cross_references":{"pubmed":["41013183"],"doi":["10.1186/s12864-025-11887-1"]}}