biostudies-literatureKe YHubei Provincial Natural Science Foundation and Traditional Chinese Medicine Innovation Joint Founding Project of Chinathe National Natural Science Foundation of China205https://www.ebi.ac.uk/biostudies/studies/S-EPMC12465971biostudies-literatureUnknown18(1)<h4>Background</h4>Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that severely impairs female fertility. Zuogui pill (ZGP) is clinically used for the treatment of reproductive disorders such as menopause syndrome and premature ovarian failure, improving female reproductive health. However, the mechanism of its action in treating PCOS remains unclear. This study aimed to investigate the mechanism of ZGP in treating PCOS based on liquid chromatography-mass spectrometry (LC-MS) and proteomics.<h4>Methods</h4>LC-MS was utilized to analyze and identify the active components of ZGP. A PCOS rat model was established using a high-fat diet combined with letrozole to assess the efficacy of ZGP. Serum hormone levels in rats were detected using ELISA, and ovarian morphoJournal of ovarian researchMechanism of Zuogui pill in the treatment of polycystic ovary syndrome based on LC-MS and proteomics.PMC12465971No.824743832023AFD114Ke YZhao MXiao MHe JTang YfalseMechanism of Zuogui pill in the treatment of polycystic ovary syndrome based on LC-MS and proteomics.<h4>Background</h4>Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that severely impairs female fertility. Zuogui pill (ZGP) is clinically used for the treatment of reproductive disorders such as menopause syndrome and premature ovarian failure, improving female reproductive health. However, the mechanism of its action in treating PCOS remains unclear. This study aimed to investigate the mechanism of ZGP in treating PCOS based on liquid chromatography-mass spectrometry (LC-MS) and proteomics.<h4>Methods</h4>LC-MS was utilized to analyze and identify the active components of ZGP. A PCOS rat model was established using a high-fat diet combined with letrozole to assess the efficacy of ZGP. Serum hormone levels in rats were detected using ELISA, and ovarian morpho2025-01-01T00:00:00Z2025 Sep2026-06-03T21:45:04.595Z2026-05-02T03:08:30.97ZS-EPMC124659714101351810.1186/s13048-025-01802-3