biostudies-literatureUnknown12Wang HAs the most feasible method to reconstruct long-distance peripheral nerve injuries, tissue-engineered nerves rely on biomaterials as a key driving factor. Chitooligosaccharides, intermediate products of chitosan degradation, have the ability to positively regulate nerve regeneration microenvironments. However, the impact of chitooligosaccharides on clearance of myelin debris during Wallerian degeneration is unrevealed. The focus is on exploring the role of chitooligosaccharides in myelin clearance, which is a crucial preparation stage for nerve regeneration. The effects of chitooligosaccharides on nerve regeneration were demonstrated through the morphological and functional evaluations. Then, the myelin lipids and proteins were analyzed using the morphological staining, and molecular and pRegenerative biomaterialsrbaf044https://www.ebi.ac.uk/biostudies/studies/S-EPMC12466441biostudies-literatureChitooligosaccharides accelarate myelin clearance by Wipi1 mediated Schwann cell autophagy promoting peripheral nerve regeneration.PMC12466441Liu JYang YLiu MZhang MWang HGong JYin LZhao YfalseChitooligosaccharides accelarate myelin clearance by Wipi1 mediated Schwann cell autophagy promoting peripheral nerve regeneration.As the most feasible method to reconstruct long-distance peripheral nerve injuries, tissue-engineered nerves rely on biomaterials as a key driving factor. Chitooligosaccharides, intermediate products of chitosan degradation, have the ability to positively regulate nerve regeneration microenvironments. However, the impact of chitooligosaccharides on clearance of myelin debris during Wallerian degeneration is unrevealed. The focus is on exploring the role of chitooligosaccharides in myelin clearance, which is a crucial preparation stage for nerve regeneration. The effects of chitooligosaccharides on nerve regeneration were demonstrated through the morphological and functional evaluations. Then, the myelin lipids and proteins were analyzed using the morphological staining, and molecular and p2025-01-01T00:00:00Z20252026-06-03T22:41:56.459Z2026-05-31T03:05:51.867ZS-EPMC124664414101892510.1093/rb/rbaf044