{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Abril D"],"funding":["Universidad El Bosque","Ministerio de Ciencia, Tecnología e Innovación","Universidad de la Sabana","Ministerio de Ciencia, Tecnologia e Innovacion of Colombia-MinCiencias","Universidad Nacional de Colombia","Fundación Santa Fe de Bogotá"],"pagination":["947"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12466754"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(9)"],"pubmed_abstract":["Carbapenem-resistant Pseudomonas aeruginosa is a major cause of healthcare associated infections in hospitalized patients and what is more warring with reduced therapeutic options. The KPC is a powerful enzyme capable of hydrolyzing the carbapenems, described first in Klebsiella pneumoniae and it already has found in P. aeruginosa.Objective: To perform a comparative genomic analysis of two new genetic platforms mobilizing the blaKPC-2 and blaKPC-3 in two ST111 and ST235 pandemic clones of P. aeruginosa in Colombia, South America. Methods: Sixty-six blaKPC-harboring P. aeruginosa isolates were identified and characterized during a prospective study conducted in six high complex hospitals in Colombia. Genetic platforms mobilizing the blaKPC were analyzed. Results: The blaKPC-2 and blaKPC-3 were identified in 24 and 42 isolates, respectively. The blaKPC-2-harboring isolates belonged to ST235 and blaKPC-3 to ST111. The whole genome sequencing indicated that the blaKPC-3 gene was mobilized by the Tn4401b within a 55-kb-size environmental origin plasmid, which, in other isolates, was inserted into the chromosome through a transposition event of ISPa38. Regarding the blaKPC-2 gene, this was mobilized by a new Non-Tn4401 Element (NTE) derived from transposon Tn2 (proposed as variant IIg), which has been transposed into a 43-Kb-size little-studied plasmid related to Klebsiella spp. Conclusions: Our results reveal a new acquisition event of blaKPC in P. aeruginosa, in this case blaKPC-3. Likewise, the pandemic high-risk clones ST111 and ST235 of P. aeruginosa continues to spread blaKPC gene through different mobile genetic elements, jumping of conventional Tn4401b and acquiring new Tn2-derived NTE, which were inserted in diverse plasmids."],"journal":["Antibiotics (Basel, Switzerland)"],"pubmed_title":["New Insights in <i>bla</i><sub>KPC</sub> Gene Mobilization in <i>Pseudomonas aeruginosa</i>: Acquisition of <i>bla</i><sub>KPC-3</sub> and Identification of a New Tn<i>2</i>-like NTE Mobilizing <i>bla</i><sub>KPC-2</sub>."],"pmcid":["PMC12466754"],"funding_grant_id":["1308-77758007","605-2018","MED-213-2016"],"pubmed_authors":["Saavedra-Trujillo CH","Gomez NV","Escobar-Perez J","Garcia JC","Bustos RH","Leal-Castro AL","Madronero J","Marquez-Ortiz RA","Bravo-Ojeda J","Rozo ZLC","Abril D"],"additional_accession":[]},"is_claimable":false,"name":"New Insights in <i>bla</i><sub>KPC</sub> Gene Mobilization in <i>Pseudomonas aeruginosa</i>: Acquisition of <i>bla</i><sub>KPC-3</sub> and Identification of a New Tn<i>2</i>-like NTE Mobilizing <i>bla</i><sub>KPC-2</sub>.","description":"Carbapenem-resistant Pseudomonas aeruginosa is a major cause of healthcare associated infections in hospitalized patients and what is more warring with reduced therapeutic options. The KPC is a powerful enzyme capable of hydrolyzing the carbapenems, described first in Klebsiella pneumoniae and it already has found in P. aeruginosa.Objective: To perform a comparative genomic analysis of two new genetic platforms mobilizing the blaKPC-2 and blaKPC-3 in two ST111 and ST235 pandemic clones of P. aeruginosa in Colombia, South America. Methods: Sixty-six blaKPC-harboring P. aeruginosa isolates were identified and characterized during a prospective study conducted in six high complex hospitals in Colombia. Genetic platforms mobilizing the blaKPC were analyzed. Results: The blaKPC-2 and blaKPC-3 were identified in 24 and 42 isolates, respectively. The blaKPC-2-harboring isolates belonged to ST235 and blaKPC-3 to ST111. The whole genome sequencing indicated that the blaKPC-3 gene was mobilized by the Tn4401b within a 55-kb-size environmental origin plasmid, which, in other isolates, was inserted into the chromosome through a transposition event of ISPa38. Regarding the blaKPC-2 gene, this was mobilized by a new Non-Tn4401 Element (NTE) derived from transposon Tn2 (proposed as variant IIg), which has been transposed into a 43-Kb-size little-studied plasmid related to Klebsiella spp. Conclusions: Our results reveal a new acquisition event of blaKPC in P. aeruginosa, in this case blaKPC-3. Likewise, the pandemic high-risk clones ST111 and ST235 of P. aeruginosa continues to spread blaKPC gene through different mobile genetic elements, jumping of conventional Tn4401b and acquiring new Tn2-derived NTE, which were inserted in diverse plasmids.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-02T03:21:20.413Z","creation":"2026-05-02T03:12:08.597Z"},"accession":"S-EPMC12466754","cross_references":{"pubmed":["41009925"],"doi":["10.3390/antibiotics14090947"]}}