biostudies-literatureChen YNational Natural Science Foundation of China1072https://www.ebi.ac.uk/biostudies/studies/S-EPMC12466801biostudies-literatureUnknown14(9)The present study aims to optimize the extraction process and systematically investigate the bioactivity of polysaccharides derived from <i>Peristrophe roxburghiana</i> (Schult.) Brem. (CPPRs). To this end, the Box-Behnken design-response surface methodology was employed to optimize the extraction parameters of polysaccharides. The optimal extraction conditions were as follows: extraction temperature, 84 °C; extraction duration, 208 min; liquid-to-material ratio, 1:27 g/mL; extraction times, 4 times. The maximum extraction yield reached 17.89%, and the yield under non-optimal extraction conditions is 11-16%. This study systematically investigated the polysaccharides' physicochemical, structural, and morphological properties using multiple advanced techniques (FTIR, SEM, XRD, HPLC, rheologyAntioxidants (Basel, Switzerland)Immune Enhancement Effects and Extraction Optimization of Polysaccharides from <i>Peristrophe roxburghiana</i>.PMC1246680132402086Li FZhan QChen YZhao ZXu YfalseImmune Enhancement Effects and Extraction Optimization of Polysaccharides from <i>Peristrophe roxburghiana</i>.The present study aims to optimize the extraction process and systematically investigate the bioactivity of polysaccharides derived from <i>Peristrophe roxburghiana</i> (Schult.) Brem. (CPPRs). To this end, the Box-Behnken design-response surface methodology was employed to optimize the extraction parameters of polysaccharides. The optimal extraction conditions were as follows: extraction temperature, 84 °C; extraction duration, 208 min; liquid-to-material ratio, 1:27 g/mL; extraction times, 4 times. The maximum extraction yield reached 17.89%, and the yield under non-optimal extraction conditions is 11-16%. This study systematically investigated the polysaccharides' physicochemical, structural, and morphological properties using multiple advanced techniques (FTIR, SEM, XRD, HPLC, rheology2025-01-01T00:00:00Z2025 Sep2026-05-02T03:08:58.783Z2026-05-02T03:07:33.944ZS-EPMC124668014100897810.3390/antiox14091072