biostudies-literatureJi KAgricultural Science and Technology Innovation Project of Shandong Academy of Agricultural SciencesNational Natural Science Foundation of ChinaKey Research and Development Program of Shandong ProvinceInnovation Team of Shandong Province Cattle Industry Technology System2733https://www.ebi.ac.uk/biostudies/studies/S-EPMC12466826biostudies-literatureUnknown15(18)In this study, we aimed to characterize intramuscular fat (IMF) tissue in fattening steers through a comparison with subcutaneous fat (SCF) tissue. The IMF of the <i>longissimus thoracis et lumborum</i> and the SCF of the back fat from three fattening steers (mean body weight of 703.50 ± 11.45 kg) were collected, and the muscle tissue, connective tissue, and fascia were carefully removed. Gene and protein expressions and the lipid contents were assessed via transcriptomic, proteomic, and lipidomic analyses, respectively. Subsequently, tissue-specific factors were identified using integrated analysis. The results revealed that the expressions of sarcoplasmic/endoplasmic reticulum Ca²⁺ transporting 2 (ATP2A2), enolase 3 (ENO3), fructose-bisphosphatase 2 (FBP2), myosin heavy chain Animals : an open access journal from MDPIComparative Analyses of Gene and Protein Expressions and the Lipid Contents in Intramuscular and Subcutaneous Fat Tissues in Fattening Steers.PMC1246682632402791SDAIT-09-04CXGC2025C10 and CXGC2025G062024TZXD031Tan ZYang MZhang XJi KZhao HfalseComparative Analyses of Gene and Protein Expressions and the Lipid Contents in Intramuscular and Subcutaneous Fat Tissues in Fattening Steers.In this study, we aimed to characterize intramuscular fat (IMF) tissue in fattening steers through a comparison with subcutaneous fat (SCF) tissue. The IMF of the <i>longissimus thoracis et lumborum</i> and the SCF of the back fat from three fattening steers (mean body weight of 703.50 ± 11.45 kg) were collected, and the muscle tissue, connective tissue, and fascia were carefully removed. Gene and protein expressions and the lipid contents were assessed via transcriptomic, proteomic, and lipidomic analyses, respectively. Subsequently, tissue-specific factors were identified using integrated analysis. The results revealed that the expressions of sarcoplasmic/endoplasmic reticulum Ca²⁺ transporting 2 (ATP2A2), enolase 3 (ENO3), fructose-bisphosphatase 2 (FBP2), myosin heavy chain 2025-01-01T00:00:00Z2025 Sep2026-05-02T03:13:40.088Z2026-05-02T03:07:57.932ZS-EPMC124668264100797810.3390/ani15182733