{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(39)"],"submitter":["Gao Q"],"pubmed_abstract":["The clinical application of CRISPR-Cas9 remains limited by delivery challenges, particularly for oral administration. Lysine-specific demethylase 1 (<i>Lsd1</i>) plays a key role in colonic inflammation and tumorigenesis. Here, we developed an oral genome-editing platform (TPGS-RNP@LNP), where <i>Lsd1</i>-targeting ribonucleoproteins (RNPs) were encapsulated in mulberry leaf lipid nanoparticles (LNPs) and formulated with d-α-tocopherol polyethylene glycol succinate (TPGS). TPGS reinforced the lipid bilayer of LNPs, enhanced gastrointestinal stability, and facilitated colonic mucus penetration. Upon the galactose receptor-mediated endocytosis of TPGS-RNP@LNPs by macrophages, their fusion with the endosomal membrane and the presence of nuclear localization signals ensured the nuclear deliver"],"journal":["Science advances"],"pagination":["eadw9275"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12466914"],"repository":["biostudies-literature"],"pubmed_title":["Reinforced plant-derived lipid nanoparticles for oral precise epigenome editing in colonic diseases."],"pmcid":["PMC12466914"],"pubmed_authors":["Zu M","Ma S","Zhu Z","Yang K","Kundu SC","Ma Y","Reis RL","Xu H","Shi X","Gao Q","Cao Y","Gao Y","Han H","Yang Q","Liu C","Xiao B"],"additional_accession":[]},"is_claimable":false,"name":"Reinforced plant-derived lipid nanoparticles for oral precise epigenome editing in colonic diseases.","description":"The clinical application of CRISPR-Cas9 remains limited by delivery challenges, particularly for oral administration. Lysine-specific demethylase 1 (<i>Lsd1</i>) plays a key role in colonic inflammation and tumorigenesis. Here, we developed an oral genome-editing platform (TPGS-RNP@LNP), where <i>Lsd1</i>-targeting ribonucleoproteins (RNPs) were encapsulated in mulberry leaf lipid nanoparticles (LNPs) and formulated with d-α-tocopherol polyethylene glycol succinate (TPGS). TPGS reinforced the lipid bilayer of LNPs, enhanced gastrointestinal stability, and facilitated colonic mucus penetration. Upon the galactose receptor-mediated endocytosis of TPGS-RNP@LNPs by macrophages, their fusion with the endosomal membrane and the presence of nuclear localization signals ensured the nuclear deliver","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-03T21:23:46.33Z","creation":"2026-05-01T03:11:08.237Z"},"accession":"S-EPMC12466914","cross_references":{"pubmed":["41004579"],"doi":["10.1126/sciadv.adw9275"]}}