{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen B"],"funding":["Science and Technology Facilities Council","Wellcome Trust","Diamond Light Source","Biotechnology and Biological Sciences Research Council"],"pagination":["1315"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12466997"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(9)"],"pubmed_abstract":["Detergent solubilisation remains the most commonly used but potentially problematic method to extract membrane proteins from lipid bilayers for Cryo-EM studies. Although recent advances have introduced excellent alternatives-such as amphipols, nanodiscs and SMALPs-the use of detergents is often necessary for intermediate steps. In this paper, we share our experiences working with detergent-solubilised samples within the modern Cryo-EM structural pipeline from the perspective of an EM specialist. Our aim is to inform novice users about potential challenges they may encounter. Drawing on specific examples from a variety of biological membrane systems, including Magnesium channels, lipopolysaccharide biosynthesis, and the human major facilitator superfamily transporters, we describe how the i"],"journal":["Biomolecules"],"pubmed_title":["Coming Clean and Avoiding Bubble Trouble-Using Detergents Wisely in the Purification of Membrane Proteins for Cryo-EM Studies."],"pmcid":["PMC12466997"],"funding_grant_id":["223727/Z/21/Z","NR-27436 & NT-33941","NR-27436 & NT-33941","ALERT 2020 BB/T017643/1","BB/T017643/1","BAG 29255 & 36408","BAG 29255 & 36408"],"pubmed_authors":["Ford RC","Harrison P","Kargas V","Collins RF","Pollock N","Prince SM","Chen B"],"additional_accession":[]},"is_claimable":false,"name":"Coming Clean and Avoiding Bubble Trouble-Using Detergents Wisely in the Purification of Membrane Proteins for Cryo-EM Studies.","description":"Detergent solubilisation remains the most commonly used but potentially problematic method to extract membrane proteins from lipid bilayers for Cryo-EM studies. Although recent advances have introduced excellent alternatives-such as amphipols, nanodiscs and SMALPs-the use of detergents is often necessary for intermediate steps. In this paper, we share our experiences working with detergent-solubilised samples within the modern Cryo-EM structural pipeline from the perspective of an EM specialist. Our aim is to inform novice users about potential challenges they may encounter. Drawing on specific examples from a variety of biological membrane systems, including Magnesium channels, lipopolysaccharide biosynthesis, and the human major facilitator superfamily transporters, we describe how the i","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-30T03:22:51.077Z","creation":"2026-06-30T03:15:54.121Z"},"accession":"S-EPMC12466997","cross_references":{"pubmed":["41008622"],"doi":["10.3390/biom15091315"]}}