biostudies-literatureLiu WNatural Science Foundation of Tianjin Science and Technology CorrespondentNational Natural Science Foundation of China1111https://www.ebi.ac.uk/biostudies/studies/S-EPMC12467003biostudies-literatureUnknown14(9)Cytochrome P450 monooxygenases (P450s) are one of the most widespread enzyme superfamilies in the biological world. In insects, P450 proteins play a crucial role in the synthesis of endogenous substances and the metabolism of xenobiotics. To date, extensive research has been conducted on P450 gene-mediated detoxification and metabolism across multiple insect species. While Chironomidae species-dominant benthic organisms inhabiting diverse ecological niches and playing critical ecological roles-remain largely uncharacterized in terms of P450 protein annotation, with the exception of a single study on <i>Propsilocerus akamusi</i>. In this study, we expanded the annotation scope by identifying the P450 protein genes in eight additional Chironomidae species. A total of 577 P450 protein genes wBiologyFunctional Annotation and Comparative Analysis of Cytochrome P450 Protein Family Genes in Nine Chironomidae Species.PMC1246700332370489, 3217047323KPHDRC00240, 22KPXMRC00070Zhou AShao ZZhang YNie JNie ZYan CLiu CWang YGao SLiu WfalseFunctional Annotation and Comparative Analysis of Cytochrome P450 Protein Family Genes in Nine Chironomidae Species.Cytochrome P450 monooxygenases (P450s) are one of the most widespread enzyme superfamilies in the biological world. In insects, P450 proteins play a crucial role in the synthesis of endogenous substances and the metabolism of xenobiotics. To date, extensive research has been conducted on P450 gene-mediated detoxification and metabolism across multiple insect species. While Chironomidae species-dominant benthic organisms inhabiting diverse ecological niches and playing critical ecological roles-remain largely uncharacterized in terms of P450 protein annotation, with the exception of a single study on <i>Propsilocerus akamusi</i>. In this study, we expanded the annotation scope by identifying the P450 protein genes in eight additional Chironomidae species. A total of 577 P450 protein genes w2025-01-01T00:00:00Z2025 Aug2026-05-02T03:20:49.52Z2026-05-02T03:12:02.382ZS-EPMC124670034100725710.3390/biology14091111