{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(9)"],"submitter":["Maksin IV"],"pubmed_abstract":["Lateral flow immunoassays (LFAs) are widely recognized as a powerful and versatile analytical platform. Nevertheless, the development of multiplex formats remains a distinct challenge. The aim of this study was to develop a multiplex LFA using gold nanoparticles (GNPs) as a label, selected for their ease of synthesis and functionalization with biomolecules. We provide practical recommendations regarding protein-hapten synthesis, membrane selection, application buffer composition, and methods to improve the long-term stability of the freeze-dried gold conjugate. The developed assay shows good tolerance to high-fat milk, stability at elevated temperatures, and promising sensitivity, with visual detection limits of 4-100 ng/mL for β-lactams, 1-10 ng/mL for tetracyclines, 50 ng/mL for streptomycin, and 0.3 ng/mL for chloramphenicol."],"journal":["Biosensors"],"pagination":["592"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12467014"],"repository":["biostudies-literature"],"pubmed_title":["Development of a Multiplex Lateral Flow Immunoassay for the Detection of Antibiotics in Milk Utilizing Lyophilized Gold Nanoparticle Conjugates."],"pmcid":["PMC12467014"],"pubmed_authors":["Kesareva VA","Kuandykova A","Polyakova DI","Khunteev GA","Maksin IV","Kirillova YG","Simonova EI","Ivanov VS","Luzyanin TA"],"additional_accession":[]},"is_claimable":false,"name":"Development of a Multiplex Lateral Flow Immunoassay for the Detection of Antibiotics in Milk Utilizing Lyophilized Gold Nanoparticle Conjugates.","description":"Lateral flow immunoassays (LFAs) are widely recognized as a powerful and versatile analytical platform. Nevertheless, the development of multiplex formats remains a distinct challenge. The aim of this study was to develop a multiplex LFA using gold nanoparticles (GNPs) as a label, selected for their ease of synthesis and functionalization with biomolecules. We provide practical recommendations regarding protein-hapten synthesis, membrane selection, application buffer composition, and methods to improve the long-term stability of the freeze-dried gold conjugate. The developed assay shows good tolerance to high-fat milk, stability at elevated temperatures, and promising sensitivity, with visual detection limits of 4-100 ng/mL for β-lactams, 1-10 ng/mL for tetracyclines, 50 ng/mL for streptomycin, and 0.3 ng/mL for chloramphenicol.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-01T03:19:23.499Z","creation":"2026-05-01T03:10:46.373Z"},"accession":"S-EPMC12467014","cross_references":{"pubmed":["41002332"],"doi":["10.3390/bios15090592"]}}