biostudies-literatureUnknown15(9)Liu Y<i>Clonorchis sinensis</i>, a parasitic liver fluke, is the primary aetiological agent of clonorchiasis, a disease predominantly characterized by liver-related clinical manifestations. Currently, research on the complete mitochondrial (mt) genome of local <i>C. sinensis</i> populations remains inadequate. Thus, in this study, we sequenced and annotated the mt genome of fish-borne <i>C. sinensis</i> (Cs-c2) from Changchun, Jilin Province, China, a strain not previously described. This mt genome is 14,136 bp in length and harbours 12 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and a single control region (CR). We constructed a maximum likelihood (ML) phylogenetic tree using concatenated <i>ND5</i>, <i>ND6</i>, and <i>ND1</i> from protein-coding genes (PCGBiomolecules1246https://www.ebi.ac.uk/biostudies/studies/S-EPMC12467321biostudies-literatureA Novel <i>Clonorchis sinensis</i> Mitogenome: Elucidating Multiregional Strain Phylogeny and Revising the Digenean Mitochondrial Genome Tree.PMC12467321Liu YZheng JZheng HChen ZZhang YWang FHu KTeng YfalseA Novel <i>Clonorchis sinensis</i> Mitogenome: Elucidating Multiregional Strain Phylogeny and Revising the Digenean Mitochondrial Genome Tree.<i>Clonorchis sinensis</i>, a parasitic liver fluke, is the primary aetiological agent of clonorchiasis, a disease predominantly characterized by liver-related clinical manifestations. Currently, research on the complete mitochondrial (mt) genome of local <i>C. sinensis</i> populations remains inadequate. Thus, in this study, we sequenced and annotated the mt genome of fish-borne <i>C. sinensis</i> (Cs-c2) from Changchun, Jilin Province, China, a strain not previously described. This mt genome is 14,136 bp in length and harbours 12 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and a single control region (CR). We constructed a maximum likelihood (ML) phylogenetic tree using concatenated <i>ND5</i>, <i>ND6</i>, and <i>ND1</i> from protein-coding genes (PCG2025-01-01T00:00:00Z2025 Aug2026-05-02T03:09:35.864Z2026-05-02T03:07:44.678ZS-EPMC124673214100855310.3390/biom15091246