biostudies-literatureBonaccorsi di Patti MCSapienza University of Rome (Progetti di Ricerca)1231https://www.ebi.ac.uk/biostudies/studies/S-EPMC12467708biostudies-literatureUnknown15(9)This study explores the structural transitions and aggregation behaviour of recombinant β- and γ-synucleins from five vertebrate species-<i>Cyprinus carpio</i>, <i>Danio rerio</i>, <i>Xenopus laevis</i>, <i>Anolis carolinensis,</i> and <i>Homo sapiens</i>-using thioflavin T fluorescence and circular dichroism spectroscopy, with and without copper ions. Although synucleins are well-conserved proteins among vertebrates, species-specific differences in amino acid composition and predicted secondary structures were observed, particularly within β-strand-forming regions. During a six-day incubation, human β-synuclein exhibited a time-dependent increase in β-sheet-rich structures, while non-mammalian β-synucleins showed limited variation. In contrast, γ-synucleins from all species displayed greaBiomoleculesComparative Analysis of Aggregation of β- and γ-Synucleins in Vertebrates.PMC12467708RM1201729CB8436FToni MMeoni MBonaccorsi di Patti MCfalseComparative Analysis of Aggregation of β- and γ-Synucleins in Vertebrates.This study explores the structural transitions and aggregation behaviour of recombinant β- and γ-synucleins from five vertebrate species-<i>Cyprinus carpio</i>, <i>Danio rerio</i>, <i>Xenopus laevis</i>, <i>Anolis carolinensis,</i> and <i>Homo sapiens</i>-using thioflavin T fluorescence and circular dichroism spectroscopy, with and without copper ions. Although synucleins are well-conserved proteins among vertebrates, species-specific differences in amino acid composition and predicted secondary structures were observed, particularly within β-strand-forming regions. During a six-day incubation, human β-synuclein exhibited a time-dependent increase in β-sheet-rich structures, while non-mammalian β-synucleins showed limited variation. In contrast, γ-synucleins from all species displayed grea2025-01-01T00:00:00Z2025 Aug2026-05-02T03:20:38.815Z2026-05-02T03:12:01.365ZS-EPMC124677084100853810.3390/biom15091231