biostudies-literatureUnknown66(12)Karouta C<h4>Purpose</h4>Dopaminergic dysregulation plays a critical role in myopia development in animal models. Although its relevance to human myopia remains uncertain, the observation that methylphenidate hydrochloride (MPH)-a dopamine (DA) and norepinephrine (NE) uptake inhibitor-slows myopia progression in children suggests a possible link. This study aimed to investigate whether MPH can inhibit myopic growth and elucidate the underlying mechanisms using an animal model.<h4>Methods</h4>MPH was administered via oral, topical, or intravitreal routes for 7 days (minimum 5 per group) to chicks undergoing form-deprivation myopia (FDM). Myopia was assessed by refraction and axial length. Retinal DA and NE dynamics-including synthesis, release, uptake, breakdown (DA only), extracellular levels, and Investigative ophthalmology & visual science52https://www.ebi.ac.uk/biostudies/studies/S-EPMC12468103biostudies-literatureMethylphenidate Inhibits the Development of Myopia by Altering Dopamine and Norepinephrine Reuptake.PMC12468103Thomson KMorgan IBooth LAshby RKarouta CfalseMethylphenidate Inhibits the Development of Myopia by Altering Dopamine and Norepinephrine Reuptake.<h4>Purpose</h4>Dopaminergic dysregulation plays a critical role in myopia development in animal models. Although its relevance to human myopia remains uncertain, the observation that methylphenidate hydrochloride (MPH)-a dopamine (DA) and norepinephrine (NE) uptake inhibitor-slows myopia progression in children suggests a possible link. This study aimed to investigate whether MPH can inhibit myopic growth and elucidate the underlying mechanisms using an animal model.<h4>Methods</h4>MPH was administered via oral, topical, or intravitreal routes for 7 days (minimum 5 per group) to chicks undergoing form-deprivation myopia (FDM). Myopia was assessed by refraction and axial length. Retinal DA and NE dynamics-including synthesis, release, uptake, breakdown (DA only), extracellular levels, and 2025-01-01T00:00:00Z2025 Sep2026-06-03T21:13:09.168Z2026-05-01T03:10:39.497ZS-EPMC124681034098580010.1167/iovs.66.12.52