{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Borile G"],"funding":["Fondazione Italiana di Ricerca per la Sclerosi Laterale Amiotrofica","Ministero dell'università e della ricerca"],"pagination":["1434"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12468161"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(18)"],"pubmed_abstract":["Neonatal rodent cardiomyocytes (CMs) are a mainstay of in vitro cardiac research, yet their immature phenotype limits the study of key physiological processes such as excitation-contraction coupling (ECC) and sympathetic modulation. Here, we present a defined low-glucose, serum-free (LGSF) culture protocol that drives the structural and functional maturation of neonatal CMs and supports their integration into functional neuro-cardiac co-cultures. After 15 days in LGSF conditions, CMs exhibit elongated morphology, organized sarcomeres, polarized connexin-43, mitochondrial redistribution, and sarcoplasmic reticulum (SR) development, all closely resembling features of adult cells. These structural hallmarks were paralleled by enhanced Ca<sup>2</sup><sup>+</sup> handling, with increased SR con"],"journal":["Cells"],"pubmed_title":["Defined Composition of Culture Media Promotes Rodent Neonatal Cardiomyocyte Maturation and Enables Functional Neuro-Cardiac Co-Culture."],"pmcid":["PMC12468161"],"funding_grant_id":["PRIN 2022F3NENH","PRIN 20229PX74A","SYMP-ALS"],"pubmed_authors":["Faggian G","Sanchez-Alonso JL","Campo A","Dokshokova L","Zaglia T","Borile G","Prando V","Mongillo M","Moro N","Gorelik J"],"additional_accession":[]},"is_claimable":false,"name":"Defined Composition of Culture Media Promotes Rodent Neonatal Cardiomyocyte Maturation and Enables Functional Neuro-Cardiac Co-Culture.","description":"Neonatal rodent cardiomyocytes (CMs) are a mainstay of in vitro cardiac research, yet their immature phenotype limits the study of key physiological processes such as excitation-contraction coupling (ECC) and sympathetic modulation. Here, we present a defined low-glucose, serum-free (LGSF) culture protocol that drives the structural and functional maturation of neonatal CMs and supports their integration into functional neuro-cardiac co-cultures. After 15 days in LGSF conditions, CMs exhibit elongated morphology, organized sarcomeres, polarized connexin-43, mitochondrial redistribution, and sarcoplasmic reticulum (SR) development, all closely resembling features of adult cells. These structural hallmarks were paralleled by enhanced Ca<sup>2</sup><sup>+</sup> handling, with increased SR con","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-01T03:19:30.044Z","creation":"2026-05-01T03:10:47.449Z"},"accession":"S-EPMC12468161","cross_references":{"pubmed":["41002399"],"doi":["10.3390/cells14181434"]}}