{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen K"],"funding":["The UPMC Children's Hospital of Pittsburgh Foundation","NIH HHS","UPMC Children's Hospital of Pittsburgh Research Advisory Committee","The Rally Foundation"],"pagination":["3002"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12468702"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(18)"],"pubmed_abstract":["<b>Background/Objectives</b>: Hepatoblastoma (HB), the most common pediatric liver cancer, often bears mutations in and/or otherwise deregulates the oncogenic transcription factors β-catenin (B), YAP (Y) and NRF2 (N). HB research is hampered by a paucity of established cell lines, particularly those possessing these molecular drivers. All combinations of B, Y and N (BY, BN, YN and BYN) are tumorigenic when overexpressed in murine livers, but it has not been possible to establish cell lines from primary tumors. Recently, we found that concurrent, in vivo Crispr-mediated targeting of the <i>Cdkn2a</i> tumor suppressor locus allows for immortalized cell lines to be efficiently generated. <b>Methods</b>: We derived and characterized five immortalized cell lines from <i>Cdkn2a</i>-targeted BN a"],"journal":["Cancers"],"pubmed_title":["Derivation of Genetically Defined Murine Hepatoblastoma Cell Lines with Angiogenic Potential."],"pmcid":["PMC12468702"],"funding_grant_id":["S10OD028483","S10 OD028483","EVP"],"pubmed_authors":["Chen K","Henchy C","Lu J","Toksoz A","Knapp J","Wang H","Prochownik EV","Ranganathan S"],"additional_accession":[]},"is_claimable":false,"name":"Derivation of Genetically Defined Murine Hepatoblastoma Cell Lines with Angiogenic Potential.","description":"<b>Background/Objectives</b>: Hepatoblastoma (HB), the most common pediatric liver cancer, often bears mutations in and/or otherwise deregulates the oncogenic transcription factors β-catenin (B), YAP (Y) and NRF2 (N). HB research is hampered by a paucity of established cell lines, particularly those possessing these molecular drivers. All combinations of B, Y and N (BY, BN, YN and BYN) are tumorigenic when overexpressed in murine livers, but it has not been possible to establish cell lines from primary tumors. Recently, we found that concurrent, in vivo Crispr-mediated targeting of the <i>Cdkn2a</i> tumor suppressor locus allows for immortalized cell lines to be efficiently generated. <b>Methods</b>: We derived and characterized five immortalized cell lines from <i>Cdkn2a</i>-targeted BN a","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-02T03:13:20.176Z","creation":"2026-05-02T03:07:55.55Z"},"accession":"S-EPMC12468702","cross_references":{"pubmed":["41008846"],"doi":["10.3390/cancers17183002"]}}