biostudies-literatureChen KThe UPMC Children's Hospital of Pittsburgh FoundationNIH HHSUPMC Children's Hospital of Pittsburgh Research Advisory CommitteeThe Rally Foundation3002https://www.ebi.ac.uk/biostudies/studies/S-EPMC12468702biostudies-literatureUnknown17(18)<b>Background/Objectives</b>: Hepatoblastoma (HB), the most common pediatric liver cancer, often bears mutations in and/or otherwise deregulates the oncogenic transcription factors β-catenin (B), YAP (Y) and NRF2 (N). HB research is hampered by a paucity of established cell lines, particularly those possessing these molecular drivers. All combinations of B, Y and N (BY, BN, YN and BYN) are tumorigenic when overexpressed in murine livers, but it has not been possible to establish cell lines from primary tumors. Recently, we found that concurrent, in vivo Crispr-mediated targeting of the <i>Cdkn2a</i> tumor suppressor locus allows for immortalized cell lines to be efficiently generated. <b>Methods</b>: We derived and characterized five immortalized cell lines from <i>Cdkn2a</i>-targeted BN aCancersDerivation of Genetically Defined Murine Hepatoblastoma Cell Lines with Angiogenic Potential.PMC12468702S10OD028483S10 OD028483EVPChen KHenchy CLu JToksoz AKnapp JWang HProchownik EVRanganathan SfalseDerivation of Genetically Defined Murine Hepatoblastoma Cell Lines with Angiogenic Potential.<b>Background/Objectives</b>: Hepatoblastoma (HB), the most common pediatric liver cancer, often bears mutations in and/or otherwise deregulates the oncogenic transcription factors β-catenin (B), YAP (Y) and NRF2 (N). HB research is hampered by a paucity of established cell lines, particularly those possessing these molecular drivers. All combinations of B, Y and N (BY, BN, YN and BYN) are tumorigenic when overexpressed in murine livers, but it has not been possible to establish cell lines from primary tumors. Recently, we found that concurrent, in vivo Crispr-mediated targeting of the <i>Cdkn2a</i> tumor suppressor locus allows for immortalized cell lines to be efficiently generated. <b>Methods</b>: We derived and characterized five immortalized cell lines from <i>Cdkn2a</i>-targeted BN a2025-01-01T00:00:00Z2025 Sep2026-05-02T03:13:20.176Z2026-05-02T03:07:55.55ZS-EPMC124687024100884610.3390/cancers17183002