{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zheng Y"],"funding":["NINDS NIH HHS","United States Department of Defense","Craig H Neilsen Foundation","NIH HHS"],"pagination":["8940"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12469384"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(18)"],"pubmed_abstract":["Human induced pluripotent stem cells (hiPSCs) hold great potential for patient-specific therapies. Transplantation of hiPSC-derived neural progenitor cells (NPCs) is a promising reparative strategy for spinal cord injury (SCI), but clinical translation requires efficient differentiation into desired neural lineages and purification before transplantation. Here, differentiated hiPSCs-reprogrammed from human skin fibroblasts using Sendai virus-mediated expression of OCT4, SOX2, KLF4, and C-MYC-into neural rosettes expressing SOX1 and PAX6, followed by neuronal precursors (β-tubulin III<sup>+</sup>/NESTIN<sup>+</sup>) and glial precursors (GFAP+/NESTIN+). Both neuronal and glial precursors expressed the A2B5 surface antigen. A2B5+ NPCs, purified by fluorescence-activated cell sorting (FACS), "],"journal":["International journal of molecular sciences"],"pubmed_title":["Functional Recovery by Transplantation of Human iPSC-Derived A2B5 Positive Neural Progenitor Cell After Spinal Cord Injury in Mice."],"pmcid":["PMC12469384"],"funding_grant_id":["R01 NS061975","385446","338617","1R01NS110707-01","R01 NS110707","1R01NS099635-01","HT9425-25-1-0376"],"pubmed_authors":["Kim DH","Xia X","Liu Y","Zheng Y","Chen X","Bu P","Zhou H","Xue H","Cao Q"],"additional_accession":[]},"is_claimable":false,"name":"Functional Recovery by Transplantation of Human iPSC-Derived A2B5 Positive Neural Progenitor Cell After Spinal Cord Injury in Mice.","description":"Human induced pluripotent stem cells (hiPSCs) hold great potential for patient-specific therapies. Transplantation of hiPSC-derived neural progenitor cells (NPCs) is a promising reparative strategy for spinal cord injury (SCI), but clinical translation requires efficient differentiation into desired neural lineages and purification before transplantation. Here, differentiated hiPSCs-reprogrammed from human skin fibroblasts using Sendai virus-mediated expression of OCT4, SOX2, KLF4, and C-MYC-into neural rosettes expressing SOX1 and PAX6, followed by neuronal precursors (β-tubulin III<sup>+</sup>/NESTIN<sup>+</sup>) and glial precursors (GFAP+/NESTIN+). Both neuronal and glial precursors expressed the A2B5 surface antigen. A2B5+ NPCs, purified by fluorescence-activated cell sorting (FACS), ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-02T03:17:28.123Z","creation":"2026-05-02T03:11:25.776Z"},"accession":"S-EPMC12469384","cross_references":{"pubmed":["41009511"],"doi":["10.3390/ijms26188940"]}}