{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Angelis KJ"],"funding":["Czech Science Foundation"],"pagination":["1091"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12469541"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(9)"],"pubmed_abstract":["<b>Background/Objectives</b>: The Structural Maintenance of Chromosomes (SMC) proteins form essential heterocomplexes for the preservation of DNA structure and its functions, and hence cell viability. The SMC5/6 dimer is assembled by direct interactions of ATP heads via the kleisin NSE4 bridge and by SMC hinges. The structure might be interrupted by a single point mutation within a conserved motif of the SMC6-hinge. We describe the phenomena associated with the impairment of the SMC5/6 complex with morphology, repair of DNA double strand breaks (DSB), mutagenesis, recombination and gene targeting (GT) in the moss <i>Physcomitrium patens</i> (<i>P. patens</i>). <b>Methods</b>: Using CRISPR/Cas9-directed oligonucleotide replacement, we have introduced two close G to R point mutations in the "],"journal":["Genes"],"pubmed_title":["The Phenotype of &lt;i&gt;Physcomitrium patens SMC6&lt;/i&gt; Mutant with Interrupted Hinge Interactions."],"pmcid":["PMC12469541"],"funding_grant_id":["GA23-05284S)","GA20-05095S"],"pubmed_authors":["Hola M","Palecek JJ","Angelis KJ","Vaculikova J","Vagnerova R"],"additional_accession":[]},"is_claimable":false,"name":"The Phenotype of &lt;i&gt;Physcomitrium patens SMC6&lt;/i&gt; Mutant with Interrupted Hinge Interactions.","description":"<b>Background/Objectives</b>: The Structural Maintenance of Chromosomes (SMC) proteins form essential heterocomplexes for the preservation of DNA structure and its functions, and hence cell viability. The SMC5/6 dimer is assembled by direct interactions of ATP heads via the kleisin NSE4 bridge and by SMC hinges. The structure might be interrupted by a single point mutation within a conserved motif of the SMC6-hinge. We describe the phenomena associated with the impairment of the SMC5/6 complex with morphology, repair of DNA double strand breaks (DSB), mutagenesis, recombination and gene targeting (GT) in the moss <i>Physcomitrium patens</i> (<i>P. patens</i>). <b>Methods</b>: Using CRISPR/Cas9-directed oligonucleotide replacement, we have introduced two close G to R point mutations in the ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-02T03:16:11.797Z","creation":"2026-05-02T03:11:11.423Z"},"accession":"S-EPMC12469541","cross_references":{"pubmed":["41010035"],"doi":["10.3390/genes16091091"]}}