biostudies-literatureAngelis KJCzech Science Foundation1091https://www.ebi.ac.uk/biostudies/studies/S-EPMC12469541biostudies-literatureUnknown16(9)<b>Background/Objectives</b>: The Structural Maintenance of Chromosomes (SMC) proteins form essential heterocomplexes for the preservation of DNA structure and its functions, and hence cell viability. The SMC5/6 dimer is assembled by direct interactions of ATP heads via the kleisin NSE4 bridge and by SMC hinges. The structure might be interrupted by a single point mutation within a conserved motif of the SMC6-hinge. We describe the phenomena associated with the impairment of the SMC5/6 complex with morphology, repair of DNA double strand breaks (DSB), mutagenesis, recombination and gene targeting (GT) in the moss <i>Physcomitrium patens</i> (<i>P. patens</i>). <b>Methods</b>: Using CRISPR/Cas9-directed oligonucleotide replacement, we have introduced two close G to R point mutations in the GenesThe Phenotype of &lt;i&gt;Physcomitrium patens SMC6&lt;/i&gt; Mutant with Interrupted Hinge Interactions.PMC12469541GA23-05284S)GA20-05095SHola MPalecek JJAngelis KJVaculikova JVagnerova RfalseThe Phenotype of &lt;i&gt;Physcomitrium patens SMC6&lt;/i&gt; Mutant with Interrupted Hinge Interactions.<b>Background/Objectives</b>: The Structural Maintenance of Chromosomes (SMC) proteins form essential heterocomplexes for the preservation of DNA structure and its functions, and hence cell viability. The SMC5/6 dimer is assembled by direct interactions of ATP heads via the kleisin NSE4 bridge and by SMC hinges. The structure might be interrupted by a single point mutation within a conserved motif of the SMC6-hinge. We describe the phenomena associated with the impairment of the SMC5/6 complex with morphology, repair of DNA double strand breaks (DSB), mutagenesis, recombination and gene targeting (GT) in the moss <i>Physcomitrium patens</i> (<i>P. patens</i>). <b>Methods</b>: Using CRISPR/Cas9-directed oligonucleotide replacement, we have introduced two close G to R point mutations in the 2025-01-01T00:00:00Z2025 Sep2026-05-02T03:16:11.797Z2026-05-02T03:11:11.423ZS-EPMC124695414101003510.3390/genes16091091