biostudies-literatureYu HHang Yu1039https://www.ebi.ac.uk/biostudies/studies/S-EPMC12469619biostudies-literatureUnknown16(9)<b>Background/Objectives:</b> Rice production faces threats from rising temperatures, demanding thermotolerant varieties. This study characterizes transcriptomic dynamics and identifies <i>Hsp101-1</i> (<i>heat shock protein 101-1</i>)-associated gene regulatory modules in rice under reproductive-stage heat stress. <b>Methods:</b> Transcriptomics and WGCNA (weighted gene co-expression network analysis) were conducted in flag leaves and spikelets for wild-type (WT) and <i>Hsp101-1</i>-overexpressing (OE) lines under 40 °C stress at six time points (0-24 h) to reveal the change in gene expressions. <b>Results:</b> The number of DEGs (differentially expressed genes) revealed substantial pre-existing differences in WT and OE lines. Pre treatment, OE flag leaves showed 545 upregulated and 676 dGenes<i>Hsp101-1</i> Orchestrates Thermotolerance in Rice via Pre-Activated Transcriptional Networks and Modular Cross-Tissue Coordination.PMC12469619R2021YJYB30172022A1515011741Jiang LChen PLi CZhang JSun BChen WLiu QMao XLyu SYu Hfalse<i>Hsp101-1</i> Orchestrates Thermotolerance in Rice via Pre-Activated Transcriptional Networks and Modular Cross-Tissue Coordination.<b>Background/Objectives:</b> Rice production faces threats from rising temperatures, demanding thermotolerant varieties. This study characterizes transcriptomic dynamics and identifies <i>Hsp101-1</i> (<i>heat shock protein 101-1</i>)-associated gene regulatory modules in rice under reproductive-stage heat stress. <b>Methods:</b> Transcriptomics and WGCNA (weighted gene co-expression network analysis) were conducted in flag leaves and spikelets for wild-type (WT) and <i>Hsp101-1</i>-overexpressing (OE) lines under 40 °C stress at six time points (0-24 h) to reveal the change in gene expressions. <b>Results:</b> The number of DEGs (differentially expressed genes) revealed substantial pre-existing differences in WT and OE lines. Pre treatment, OE flag leaves showed 545 upregulated and 676 d2025-01-01T00:00:00Z2025 Aug2026-05-01T03:20:51.128Z2026-05-01T03:11:23.084ZS-EPMC124696194100998410.3390/genes16091039