biostudies-literatureUnknown26(18)Fraga-Garcia PWhen iron oxide nanoparticles are incubated together with a biological broth, the biomolecules compete for the binding sites at the solid-liquid interface. At the same time, the biomass rearranges in suspension, building agglomerated structures. Despite general knowledge of the forces involved in bio-nano interactions, gaps remain in the understanding of how biomolecules organize themselves in solution and onto surfaces. This work examines biomolecule adsorption onto metal oxide surfaces with the goal of strengthening this understanding, essential in industrial and natural processes. We demonstrate nearly complete separation of proteins from a biotechnological suspension for non-oxidized and highly oxidized magnetic nanoparticles. Varying the nanoparticle-to-biomass ratio, we find, can leaInternational journal of molecular sciences8995https://www.ebi.ac.uk/biostudies/studies/S-EPMC12469985biostudies-literatureBio Meets Nano: Protein Exchange in Saline Biocoronae on Magnetic Nanoparticles.PMC12469985Kube MHaßelt SKaveh-Baghbaderani YDietz HFraga-Garcia PAbarca-Cabrera LSchwaminger SPDiaz-Cano CEfalseBio Meets Nano: Protein Exchange in Saline Biocoronae on Magnetic Nanoparticles.When iron oxide nanoparticles are incubated together with a biological broth, the biomolecules compete for the binding sites at the solid-liquid interface. At the same time, the biomass rearranges in suspension, building agglomerated structures. Despite general knowledge of the forces involved in bio-nano interactions, gaps remain in the understanding of how biomolecules organize themselves in solution and onto surfaces. This work examines biomolecule adsorption onto metal oxide surfaces with the goal of strengthening this understanding, essential in industrial and natural processes. We demonstrate nearly complete separation of proteins from a biotechnological suspension for non-oxidized and highly oxidized magnetic nanoparticles. Varying the nanoparticle-to-biomass ratio, we find, can lea2025-01-01T00:00:00Z2025 Sep2026-05-01T03:21:13.761Z2026-05-01T03:11:13.373ZS-EPMC124699854100956210.3390/ijms26188995