biostudies-literatureGuo JLihua QiuLulu YanBo Zhang1012https://www.ebi.ac.uk/biostudies/studies/S-EPMC12470244biostudies-literatureUnknown16(9)<h4>Background</h4>Primordial germ cells (PGC) are the progenitor cells of sperm and eggs during the embryonic stage. The maternal gene vasa has been widely studied for its role in PGC origin, and other genes like <i>dead end</i> (dnd) have also been identified.<h4>Objectives</h4>Spotted sea bass is an important economic marine fish, and the study of its germ cell characteristics provides important basic data for future population breeding and protection.<h4>Methods</h4>In this study, we cloned the full-length sequences of <i>Lmvasa</i> (2384 bp, encoding 1905 aa) and <i>Lmdnd</i> (1523 bp, encoding 386 aa) using RACE. Temporal and spatial expression patterns of Lmvasa and Lmdnd in embryos and gonads were analyzed by PCR, immunohistochemistry, and in situ hybridization. We also used microiGenesGenetic Characterization of Primordial Germ Cells in Spotted Sea Bass (&lt;i&gt;Lateolabrax maculatus&lt;/i&gt;).PMC124702442022YFD2400503；2024CXTD272023YFD2401701; 2024-SPY-00-008No. 2024JS04；No. 2023TD21Yan LGuo JZhao CZhang BQiu LfalseGenetic Characterization of Primordial Germ Cells in Spotted Sea Bass (&lt;i&gt;Lateolabrax maculatus&lt;/i&gt;).<h4>Background</h4>Primordial germ cells (PGC) are the progenitor cells of sperm and eggs during the embryonic stage. The maternal gene vasa has been widely studied for its role in PGC origin, and other genes like <i>dead end</i> (dnd) have also been identified.<h4>Objectives</h4>Spotted sea bass is an important economic marine fish, and the study of its germ cell characteristics provides important basic data for future population breeding and protection.<h4>Methods</h4>In this study, we cloned the full-length sequences of <i>Lmvasa</i> (2384 bp, encoding 1905 aa) and <i>Lmdnd</i> (1523 bp, encoding 386 aa) using RACE. Temporal and spatial expression patterns of Lmvasa and Lmdnd in embryos and gonads were analyzed by PCR, immunohistochemistry, and in situ hybridization. We also used microi2025-01-01T00:00:00Z2025 Aug2026-05-03T03:14:46.644Z2026-05-03T03:11:00.687ZS-EPMC124702444100995810.3390/genes16091012