{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Marinescu M"],"funding":["Deutsche Arthrose-Hilfe"],"pagination":["339"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12470832"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(9)"],"pubmed_abstract":["Osteoarthritis (OA), the most common joint disease, is marked by cartilage degradation and chronic inflammation. While 45S5-bioactive glass (45S5-BG) is well-established in bone regeneration and has been suggested to exert immunomodulatory effects, its impact on OA chondrocytes remains largely unexplored. Therefore, this in vitro study investigated the effects of 45S5-BG microparticles (0.125 mg/mL) on chondrocytes derived from OA patients, evaluating its therapeutic potential in OA. Chondrocytes were cultured with or without 45S5-BG for 1 and 7 days. Gene expression of cartilage markers, cytokines, matrix metalloproteinases (MMPs), and toll-like receptors (TLRs) was analyzed by qPCR. Protein levels were assessed by ELISA. 45S5-BG stimulation significantly altered chondrocyte activity, inducing upregulation of IL-6, IL-1β, TNF-α, MMP-1/-3/-13, and TLR4. Expression of ACAN and COL2A1 was reduced, while COL10A1-a marker of chondrocyte hypertrophy-was significantly increased at day 1. These findings show a catabolic and pro-inflammatory shift in chondrocyte phenotype upon 45S5-BG exposure, showing no therapeutic benefit of 45S5-BG on OA chondrocytes. However, considering the pronounced effects on chondrocyte activity and the well-established bioactivity and biocompatibility of 45S5-BG, our findings suggest that modified BG formulations could be developed to enhance chondroprotective and anti-inflammatory properties, warranting further investigation in co-culture and in vivo models."],"journal":["Journal of functional biomaterials"],"pubmed_title":["Impact of 45S5-Bioactive Glass on Chondrocytes in Knee Osteoarthritis-In Vitro Study Exploring Cellular Responses."],"pmcid":["PMC12470832"],"funding_grant_id":["P515-A623"],"pubmed_authors":["Westhauser F","Mayakrishnan R","Moradi B","Fellenberg J","Renkawitz T","Platzer H","Hagmann S","Gantz S","Marinescu M","Boccaccini AR","Tripel E"],"additional_accession":[]},"is_claimable":false,"name":"Impact of 45S5-Bioactive Glass on Chondrocytes in Knee Osteoarthritis-In Vitro Study Exploring Cellular Responses.","description":"Osteoarthritis (OA), the most common joint disease, is marked by cartilage degradation and chronic inflammation. While 45S5-bioactive glass (45S5-BG) is well-established in bone regeneration and has been suggested to exert immunomodulatory effects, its impact on OA chondrocytes remains largely unexplored. Therefore, this in vitro study investigated the effects of 45S5-BG microparticles (0.125 mg/mL) on chondrocytes derived from OA patients, evaluating its therapeutic potential in OA. Chondrocytes were cultured with or without 45S5-BG for 1 and 7 days. Gene expression of cartilage markers, cytokines, matrix metalloproteinases (MMPs), and toll-like receptors (TLRs) was analyzed by qPCR. Protein levels were assessed by ELISA. 45S5-BG stimulation significantly altered chondrocyte activity, inducing upregulation of IL-6, IL-1β, TNF-α, MMP-1/-3/-13, and TLR4. Expression of ACAN and COL2A1 was reduced, while COL10A1-a marker of chondrocyte hypertrophy-was significantly increased at day 1. These findings show a catabolic and pro-inflammatory shift in chondrocyte phenotype upon 45S5-BG exposure, showing no therapeutic benefit of 45S5-BG on OA chondrocytes. However, considering the pronounced effects on chondrocyte activity and the well-established bioactivity and biocompatibility of 45S5-BG, our findings suggest that modified BG formulations could be developed to enhance chondroprotective and anti-inflammatory properties, warranting further investigation in co-culture and in vivo models.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-01T03:20:18.186Z","creation":"2026-05-01T03:10:57.113Z"},"accession":"S-EPMC12470832","cross_references":{"pubmed":["41003410"],"doi":["10.3390/jfb16090339"]}}