biostudies-literatureDong DOpen Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong ProvinceGuangdong Basic and Applied Basic Research FoundationNational Natural Science Foundation of China901https://www.ebi.ac.uk/biostudies/studies/S-EPMC12470858biostudies-literatureUnknown16(9)<i>Diaphorina citri</i> is the primary global vector of "<i>Candidatus</i> Liberibacter asiaticus", the bacterium responsible for Huanglongbing. Syntaxin-1A (<i>Syx1A</i>), a member of the Qa-SNARE family, is essential for vesicle fusion and signal transduction, though its function in hemipteran insects remains poorly understood. This study presents the first comprehensive analysis of <i>Syx1A</i> expression in <i>D. citri</i>. Transcripts were detected across all life stages, with peak expression in the salivary glands. RNAi silencing of <i>Syx1A</i> reduced mRNA levels by 39.0% in nymphs and 58.0% in adults, resulting in 58.3% nmortality in nymphs within 5 days and 73.3% in adults within seven days, along with significant weight loss. Treated females showed marked declines in fecundity, InsectsSyntaxin-1A Silencing by RNAi Disrupts Growth and Reproduction in the Asian Citrus Psyllid, &lt;i&gt;Diaphorina citri&lt;/i&gt;.PMC12470858324023522023SDZG062025A15150110292022A1515110401Dong DQiu BWang XChang CGuo CfalseSyntaxin-1A Silencing by RNAi Disrupts Growth and Reproduction in the Asian Citrus Psyllid, &lt;i&gt;Diaphorina citri&lt;/i&gt;.<i>Diaphorina citri</i> is the primary global vector of "<i>Candidatus</i> Liberibacter asiaticus", the bacterium responsible for Huanglongbing. Syntaxin-1A (<i>Syx1A</i>), a member of the Qa-SNARE family, is essential for vesicle fusion and signal transduction, though its function in hemipteran insects remains poorly understood. This study presents the first comprehensive analysis of <i>Syx1A</i> expression in <i>D. citri</i>. Transcripts were detected across all life stages, with peak expression in the salivary glands. RNAi silencing of <i>Syx1A</i> reduced mRNA levels by 39.0% in nymphs and 58.0% in adults, resulting in 58.3% nmortality in nymphs within 5 days and 73.3% in adults within seven days, along with significant weight loss. Treated females showed marked declines in fecundity, 2025-01-01T00:00:00Z2025 Aug2026-05-02T03:18:31.626Z2026-05-02T03:08:56.574ZS-EPMC124708584100908410.3390/insects16090901